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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Enzymatic Extraction of Pilocarpine from Pilocarpus jaborandi
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Enzymatic Extraction of Pilocarpine from Pilocarpus jaborandi

机译：毛果毛果毛果芸香碱的酶法提取

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摘要

Pilocarpine is an imidazole alkaloid, found exclusively in the Pilocarpus genus, with huge pharmaceutical importance. In order to extract pilocarpine from Pilocarpus jaborandi, environmentally friendly enzyme-assisted extraction was applied. Viscozyme~R L, a commercially available enzyme cocktail, was used for the study. The conditions for extraction were optimized on the basis of substrates, enzymes, temperatures and pHs. Optimum conditions for extraction with the highest yield were 30 h reaction of100 mg substance at 45°C in 40 ml of 50 mM acetic acid, pH 4. A 10% enzyme concentration was found to be the best for extraction. Total pilocarpine content after extraction was analyzed by HPLC. The total pilocarpine content (1.14 ug/mg) obtained fromViscozyme~R L treatment was 3.08-fold greater than those of the control treatment (0.37 mu g/mg).
机译：毛果芸香碱是一种咪唑生物碱，仅存在于毛果芸香属中，具有重要的药学意义。为了从辣椒果中提取毛果芸香碱，采用了环境友好的酶辅助提取方法。使用市售的酶混合物Viscozyme R L进行研究。根据底物，酶，温度和pH对提取条件进行了优化。最高收率的最佳提取条件是100 mg物质在45°C下于40 ml 50 mM乙酸（pH 4）中反应30 h。发现10％的酶浓度是最佳提取条件。提取后的总毛果芸香碱含量通过HPLC分析。通过Viscozyme〜RL处理获得的毛果芸香碱总含量（1.14 ug / mg）比对照处理（0.37μg/ mg）高3.08倍。

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