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首页> 外文期刊>Korean Journal of Microbiology and Biotechnology >Antimicrobial Effect of Inula britannica Flower Extract against Methicillin-resistant Staphylococcus aureus
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Antimicrobial Effect of Inula britannica Flower Extract against Methicillin-resistant Staphylococcus aureus

机译：大黄菊花提取物对耐甲氧西林金黄色葡萄球菌的抗菌作用

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摘要

The antimicrobial effect of the methanol extract of Inula britannica flowers against methicillin resistant Staphylococcus aureus (MRSA) was investigated. It was confirmed that the methanol extract is mainly composed of quercetin, which has antimicrobial properties. The antimicrobial effect of the methanol extract against 3 MRSA strains was determined by the disc diffusion method. The minimal inhibitory concentrations were ranged from 0.625 mg/ml to 1.25 mg/ml, and the minimum bactericidal concentrations were 2.5 mg/ml. Time kill kinetics revealed bactericidal activities, and the morphological alterations in S. aureus ATCC 33591 treated with the extract were observed using a scanning electron microscope. The methanol extract affected the expression of the resistant genes, mecA, mecl, and mecRI in mRNA. Therefore, the methanol extract of /. britannica flowers clearly demonstrated an antimicrobial effect against MRSA and these results suggest a potential for application as a natural antimicrobial agent.
机译：研究了大花菊的甲醇提取物对耐甲氧西林的金黄色葡萄球菌（MRSA）的抗菌作用。证实了甲醇提取物主要由槲皮素组成，其具有抗菌特性。通过圆盘扩散法确定了甲醇提取物对3种MRSA菌株的抗菌作用。最小抑菌浓度为0.625 mg / ml至1.25 mg / ml，最小抑菌浓度为2.5 mg / ml。时间杀灭动力学揭示了杀菌活性，并且使用扫描电子显微镜观察了用提取物处理的金黄色葡萄球菌ATCC 33591的形态变化。甲醇提取物影响mRNA中抗性基因mecA，mecl和mecRI的表达。因此，甲醇提取物为/。大不列颠花清楚地表明了对MRSA的抗菌作用，这些结果表明了其作为天然抗菌剂的应用潜力。

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