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首页> 外文期刊>Molecular genetics and genomics: MGG >Genome-wide identification, classification and expression analysis in fungal-plant interactions of cutinase gene family and functional analysis of a putative ClCUT7 in Curvularia lunata
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Genome-wide identification, classification and expression analysis in fungal-plant interactions of cutinase gene family and functional analysis of a putative ClCUT7 in Curvularia lunata

机译：角质酶基因家族在真菌-植物相互作用中的全基因组鉴定，分类和表达分析以及假定的弯孢霉ClCUT7的功能分析

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Cutinase is described as playing various roles in fungal-plant pathogen interactions, such as eliciting host-derived signals, fungal spore attachment and carbon acquisition during saprophytic growth. However, the characteristics of the cutinase genes, their expression in compatible interactions and their roles in pathogenesis have not been reported in Curvularia lunata, an important leaf spot pathogen of maize in China. Therefore, a cutinase gene family analysis could have profound significance. In this study, we identified 13 cutinase genes (ClCUT1 to ClCUT13) in the C. lunata genome. Multiple sequence alignment showed that most fungal cutinase proteins had one highly conserved GYSQG motif and a similar DxVCxG[ST]-[LIVMF](3)-x(3)H motif. Gene structure analyses of the cutinases revealed a complex intron-exon pattern with differences in the position and number of introns and exons. Based on phylogenetic relationship analysis, C. lunata cutinases and 78 known cutinase proteins from other fungi were classified into four groups with subgroups, but the C. lunata cutinases clustered in only three of the four groups. Motif analyses showed that each group of cutinases from C. lunata had a common motif. Real-time PCR indicated that transcript levels of the cutinase genes in a compatible interaction between pathogen and host had varied expression patterns. Interestingly, the transcript levels of ClCUT7 gradually increased during early pathogenesis with the most significant up-regulation at 3 h post-inoculation. When ClCUT7 was deleted, pathogenicity of the mutant decreased on unwounded maize (Zea mays) leaves. On wounded maize leaves, however, the mutant caused symptoms similar to the wild-type strain. Moreover, the ClCUT7 mutant had an approximately 10 % reduction in growth rate when cutin was the sole carbon source. In conclusion, we identified and characterized the cutinase family genes of C. lunata, analyzed their expression patterns in a compatible host-pathogen interaction, and explored the role of ClCUT7 in pathogenicity. This work will increase our understanding of cutinase genes in other fungal-plant pathogens.
机译：角质酶被描述为在真菌与植物病原体的相互作用中扮演各种角色，例如在腐生植物的生长过程中引发宿主衍生信号，真菌孢子附着和碳捕获。然而，尚未在中国重要的叶斑病原菌弯孢弯曲菌中报道过角质酶基因的特性，它们在相容相互作用中的表达及其在发病机理中的作用。因此，角质酶基因家族分析可能具有深远的意义。在这项研究中，我们确定了月球梭菌基因组中的13个角质酶基因（ClCUT1至ClCUT13）。多序列比对显示，大多数真菌角质酶蛋白具有一个高度保守的GYSQG基序和相似的DxVCxG [ST]-[LIVMF]（3）-x（3）H基序。角质酶的基因结构分析显示了一个复杂的内含子-外显子模式，内含子和外显子的位置和数量不同。根据系统发育关系分析，月牙果角质酶和其他真菌中的78种已知角质酶蛋白分为四个亚组，而月牙果角质酶仅聚在四组中的三组中。母题分析表明，每组来自月球梭菌的角质酶都有一个共同的基序。实时PCR表明，病原体和宿主之间相容相互作用中角质酶基因的转录水平具有不同的表达模式。有趣的是，ClCUT7的转录水平在早期发病机理中逐渐增加，在接种后3 h最明显的上调。当删除ClCUT7时，突变体在未受伤的玉米（玉米）叶上的致病性降低。然而，在受伤的玉米叶片上，该突变体引起的症状类似于野生株。此外，当角质是唯一的碳源时，ClCUT7突变体的生长速率降低了约10％。总之，我们鉴定和表征了月球梭菌的角质酶家族基因，分析了它们在相容的宿主-病原体相互作用中的表达模式，并探讨了ClCUT7在致病性中的作用。这项工作将增加我们对其他真菌植物病原体中角质酶基因的了解。

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