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首页> 外文期刊>Nano Hybrids >Fabrication of an Enzymatic Biosensor Based on Gold Nanoparticles Modified Electrochemical Transducer for the Detection of Organophosphorus Compounds
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Fabrication of an Enzymatic Biosensor Based on Gold Nanoparticles Modified Electrochemical Transducer for the Detection of Organophosphorus Compounds

机译：基于金纳米粒子修饰电化学传感器的酶生物传感器的制备，用于检测有机磷化合物

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摘要

A facile and sensitive enzyme based electrochemical transducer has been fabricated for the detection of organophosporus compounds. The enzyme, acetylcholinesterase, was covalently immobilized on gold nanoparticles deposited electrochemically over screen printed carbon working electrode. The electrodes were characterized by scanning electron microscopy, atomic force microscopy and electrochemical methods. The enzyme-substrate reactions and sensing studies were carried out at room temperature by cyclic voltammetry. The developed biosensor gave optimum response within 25 sec. for a substrate (acetylthiocholine) concentration of 0.0699 mM at pH. The electrode showed a linear response in the range between 0.2 and 1 ppb, and the detection limit was determined to be 0.6 ppb. Moreover the biosensor exhibited good reusability and stability thus, making it a promising tool for on-field detection of organophosphorus compounds.
机译：已经制造了一种简便且灵敏的基于酶的电化学传感器，用于检测有机磷化合物。该酶乙酰胆碱酯酶被共价固定在电化学沉积在丝网印刷碳工作电极上的金纳米颗粒上。通过扫描电子显微镜，原子力显微镜和电化学方法对电极进行表征。酶-底物反应和传感研究是在室温下通过循环伏安法进行的。开发的生物传感器在25秒内提供了最佳响应。 pH下底物（乙酰硫胆碱）的浓度为0.0699 mM。电极在0.2 ppb至1 ppb的范围内显示线性响应，并且检测极限确定为0.6 ppb。此外，该生物传感器显示出良好的可重复使用性和稳定性，因此使其成为有机磷化合物现场检测的有前途的工具。

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