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首页> 外文期刊>Biochemistry >Positioning of a Nucleosome on Mouse Satellite DNA Inserted into a Yeast Plasmid Is Determined by Its DNA Sequence and an Adjacent Nucleosome
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Positioning of a Nucleosome on Mouse Satellite DNA Inserted into a Yeast Plasmid Is Determined by Its DNA Sequence and an Adjacent Nucleosome

机译：核糖体在插入酵母质粒中的小鼠卫星DNA上的定位是由其DNA序列和相邻的核糖体确定的。

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It has earlier been shown that multiple positioning of nucleosomes on mouse satellite DNA is determined by its nucleotide sequence.To clarify whether other factors,such as boundary ones,can affect the positionings,we modified the environment of satellite DNA monomer by inserting it into a yeast plasmid between inducible GalCyc promoter and a structural region of the yeast FLPgene.We have revealed that the positions of nucleosomes on satellite DNA are identical to those detected upon reconstruction in vitro.The positioning signal(GAAAAA sequence)of satellite DNA governs nucleosome location at the adjacent nucleotide sequence as well.Upon promoter induction the nucleosome,translationally positioned on the GalCyc promoter,transfers to the satellite DNA and its location follows the positioning signal of the latter.Thus,the alternatives of positioning of a nucleosome on satellite DNA are controlled by its nucleotide sequence,though the choice of one of them is determined by the adjacent nucleosome.
机译：先前已经证明，核小体在小鼠卫星DNA上的多重定位是由其核苷酸序列决定的。为弄清边界等其他因素是否会影响定位，我们通过将卫星DNA单体插入分子中来修饰其环境。酵母质粒可诱导的GalCyc启动子和酵母FLP基因的结构区之间。我们发现卫星DNA上核小体的位置与体外重建时检测到的相同。卫星DNA的定位信号（GAAAAA序列）控制着核小体的位置。在启动子诱导后，翻译定位在GalCyc启动子上的核小体转移到卫星DNA上，其位置遵循后者的定位信号。因此，控制了核小体在卫星DNA上的定位选择。通过其核苷酸序列，尽管其中之一的选择取决于相邻的核苷酸脂质体。

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