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Electrochemically programmed release of biomolecules and nanoparticles

机译:电化学编程释放生物分子和纳米颗粒

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The controlled release of molecules or nanoparticle conjugates is an important tool for a wide range of applications in science and engineering. Here we demonstrate electrochemically programmed release of biomolecules and nanoparticles immobilized on patterned gold electrodes using the thiol-gold linkage. This technique exploits the reductive desorption of self-assembled monolayers and allows both spatially controlled release and regeneration of small molecules (e.g., drugs), biopolymers (e.g., peptides, proteins, DNA), protein assemblies (e.g., viruses), and nanoparticles (e.g., particle-DNA conjugates). Fluorescence microscopy is used to image the release of avidin and nanoparticles in phosphate-buffered saline and to determine the kinetics of desorption. We also demonstrate that the electrodes can be regenerated using the same conjugation scheme.
机译:分子或纳米粒子结合物的受控释放是在科学和工程中广泛应用的重要工具。在这里,我们展示了使用硫醇-金键固定在带图案的金电极上的生物分子和纳米颗粒的电化学程序化释放。这项技术利用了自组装单分子膜的还原性解吸作用,并允许小分子(例如药物),生物聚合物(例如肽,蛋白质,DNA),蛋白质组装体(例如病毒)和纳米颗粒(例如,粒子-DNA结合物)。荧光显微镜用于对抗生物素蛋白和纳米颗粒在磷酸盐缓冲液中的释放进行成像,并确定解吸动力学。我们还证明了可以使用相同的共轭方案来再生电极。

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