Detecting DNA folding with nanocapillaries

Steinbock L.J.; Otto O.; Chimerel C.; Gornall J.; Keyser U.F.

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Detecting DNA folding with nanocapillaries

机译：用纳米毛细管检测DNA折叠

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We demonstrate for the first time the detection of the folding state of double-stranded DNA in nanocapillaries with the resistive pulse technique. We show that glass capillaries can be pulled into nanocapillaries with diameters down to 45 nm. We study translocation of λ -DNA which is driven by an electrophoretic force through the nanocapillary. The resulting change in ionic current indicates the folding state of single λ -DNA molecules. Our experiments prove that nanocapillaries are suitable for label-free analysis of DNA in aqueous solutions and viable alternatives to solid-state nanopores made by silicon nanotechnology.

机译：我们首次证明了使用电阻脉冲技术检测纳米毛细管中双链DNA的折叠状态。我们表明，玻璃毛细管可以拉入直径小于45 nm的纳米毛细管中。我们研究了通过纳米毛细管电泳力驱动的λ-DNA的移位。离子电流的最终变化表明单个λ-DNA分子的折叠状态。我们的实验证明，纳米毛细管适用于水溶液中DNA的无标记分析，是硅纳米技术制得的固态纳米孔的可行替代品。

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《Nano letters》 |2010年第7期|共5页
作者
Steinbock L.J.; Otto O.; Chimerel C.; Gornall J.; Keyser U.F.;
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原文格式 PDF
正文语种 eng
中图分类特种结构材料;
关键词
DNA folding; Electrophoretic translocation; Nanocapillary; Nanopore; Sensing;

机译：DNA折叠电泳易位纳米毛细管纳米孔传感;

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专利

1. Detecting DNA folding with nanocapillaries [J] . Steinbock L.J., Otto O., Chimerel C., Nano letters . 2010,第7期

机译：用纳米毛细管检测DNA折叠
2. Clerocidin-mediated DNA footprinting discriminates among different G-quadruplex conformations and detects tetraplex folding in a duplex environment [J] . NadaiM., SattinG., PalùG., Biochimica et Biophysica Acta. General Subjects . 2013,第10期

机译：Clerocidin介导的DNA足迹可区分不同的G-四链体构象，并检测双链体环境中的四链体折叠
3. Protein folding coupled to DNA binding in the catalytic domain of bacteriophage lambda integrase detected by mass spectrometry. [J] . Kamadurai HB, Subramaniam S, Jones RB, Protein Science: A Publication of the Protein Society . 2003,第3期

机译：通过质谱检测，蛋白质折叠与DNA结合在噬菌体λ整合酶的催化域中。
4. Nanocapillaries and Optical Tweezers for Studies on DNA in Confinement [C] . Oliver Otto, Lorenz J. Steinbock, Ulrich F. Keyser Optical trapping and optical micromanipulation VIII . 2011

机译：纳米毛细管和光学镊子用于限制DNA的研究
5. Elucidating the origins of cooperativity in protein folding and detecting parallel folding pathways with consensus Ankyrin Repeat proteins [D] . Aksel, Tural 2012

机译：阐明蛋白质折叠中协同作用的起源，并通过锚蛋白重复序列蛋白检测平行折叠途径
6. Protein folding coupled to DNA binding in the catalytic domain of bacteriophage λ integrase detected by mass spectrometry [O] . Hari B. Kamadurai, Srisunder Subramaniam, R. Benjamin Jones, 2003

机译：质谱检测到蛋白质折叠与DNA结合在噬菌体λ整合酶的催化域中
7. Selective Detection of Single-Stranded DNA Molecules Using a Glass Nanocapillary Functionalized with DNA [O] . -1

机译：使用用DNA官能化的玻璃纳米咖啡瘤选择性检测单链DNA分子

Detecting DNA folding with nanocapillaries

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