Gold NanoStoves for microsecond DNA melting analysis

Stehr J; Hrelescu C; Sperling RA; Raschke G; Wunderlich M; Nichtl A; Heindl D; Kurzinger K; Parak WJ; Klar TA

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Gold NanoStoves for microsecond DNA melting analysis

机译：金NanoStoves用于微秒DNA熔解分析

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In traditional DNA melting assays, the temperature of the DNA-containing solution is slowly ramped up. In contrast, we use 300 ns laser pulses to rapidly heat DNA bound gold nanoparticle aggregates. We show that double-stranded DNA melts on a microsecond time scale that leads to a disintegration of the gold nanoparticle aggregates on a millisecond time scale. A perfectly matching and a point-mutated DNA sequence can be clearly distinguished in less than one millisecond even in a 1:1 mixture of both targets.

机译：在传统的DNA熔解测定中，含DNA溶液的温度缓慢升高。相反，我们使用300 ns的激光脉冲来快速加热结合DNA的金纳米颗粒聚集体。我们表明，双链DNA在毫秒级的时间尺度上融化，从而导致金纳米粒子聚集体在毫秒级的时间上解体。即使两个靶标以1：1的比例混合，也可以在不到一毫秒的时间内清楚地区分出完全匹配和点突变的DNA序列。

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《Nano letters》 |2008年第2期|共5页
作者
Stehr J; Hrelescu C; Sperling RA; Raschke G; Wunderlich M; Nichtl A; Heindl D; Kurzinger K; Parak WJ; Klar TA;
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正文语种 eng
中图分类特种结构材料;
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SURFACE-PLASMON RESONANCE; THERMAL-DENATURATION; OPTICAL-PROPERTIES; NANOPARTICLES; SINGLE; DIFFERENTIATION; POLYNUCLEOTIDES; HYBRIDIZATION; PARTICLES; PROBES;

机译：表面等离子体共振;热变性;光学性能;纳米颗粒;单;微分;多核苷酸;杂交;颗粒;问题;

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1. Gold NanoStoves for microsecond DNA melting analysis [J] . Stehr J, Hrelescu C, Sperling RA, Nano letters . 2008,第2期

机译：金NanoStoves用于微秒DNA熔解分析
2. DNA concentration can specify DNA melting point in a high-resolution melting analysis master mix [J] . NgJ.W.S., HoltD.C., AnderssonP., Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2014,第2期

机译：DNA浓度可以在高分辨率熔解分析预混液中指定DNA熔点
3. DNA Concentration Can Specify DNA Melting Point in a High-Resolution Melting Analysis Master Mix [J] . Deborah C. Holt, Jacklyn W.S. Ng, Patiyan Andersson, Clinical Chemistry: Journal of the American Association for Clinical Chemists . 2014,第2期

机译：DNA浓度可以在高分辨率熔解分析预混液中指定DNA熔点
4. UNCERTAINTY ANALYSIS OF MELTING AND RESOLIDIFICATION OF GOLD FILM IRRADIATED BY NANO- TO FEMTOSECOND LASERS USING STOCHASTIC METHOD [C] . Nazia Afrin, Yuwen Zhang, J. K. Chen ASME International Conference on Micro/Nanoscale Heat and Mass Transfer . 2016

机译：利用随机法测定纳米对飞秒激光器辐射金膜的熔化与调整性的不确定性分析
5. Studies of schistosome DNA: Species and strain differences from melting and drug resistance DNA analysis and cloning, sequencing and expression analysis of a putative praziquantel binding Ca(++)-ATPase gene. [D] . Tsai, Meng-Hsiun. 1998

机译：血吸虫DNA的研究：物种和菌株差异从融化和耐药性DNA分析以及推定的吡喹酮结合Ca（++）-ATPase基因的克隆，测序和表达分析。
6. Electrical detection of the temperature induced melting transition of a DNA hairpin covalently attached to gold interdigitated microelectrodes [O] . Greg P. Brewood, Yaswanth Rangineni, Daniel J. Fish, 2008

机译：电检测温度诱导的DNA发夹共价连接至金指状微电极的熔融转变
7. Highly stable and biocompatible gold nanorod–DNA conjugates as NIR probes for ultrafast sequence-selective DNA melting [O] . Verena Baumann, Peter Johan Friedrich Röttgermann, Frederik Haase, 2016

机译：高度稳定和生物相容性的金纳米棒-DNA缀合物作为超快序列选择性DNA熔化的NIR探针
8. Optical melting as a tool for optimizing SBH analysis of DNA [R] . Doktycz, M. J. , Jacobson, K. B. , Foote, R. S. , 1995

机译：光学熔融作为优化DNa的sBH分析的工具

Gold NanoStoves for microsecond DNA melting analysis

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