Measurement of glucose, sucrose and lactose in food samples with enzyme-immobilised packed-bed column reactors integrated to an amperometric enzyme electrode

Boyaci IH; Mutlu M

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Measurement of glucose, sucrose and lactose in food samples with enzyme-immobilised packed-bed column reactors integrated to an amperometric enzyme electrode

机译：使用固定在安培酶电极上的酶固定式填充柱反应器测量食品中的葡萄糖，蔗糖和乳糖

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An amperometric enzyme electrode integrated with packed-bed column reactors containing immobilised enzymes was improved to measure glucose, sucrose and lactose content of food samples with only one sampling. Performance parameters of the system were investigated on the bases of linearity, sensitivity and response time. Those values were found to be 20 mm, 24.9 nA/mM and 20 s for glucose; 20 mm, 12.0 nA/mM and 3 min for sucrose; 12 mm, 10.8 nA/mM and 4 min for lactose, respectively. Overall analysis time for three analytes with one sampling was less than 8 min without pretreatment and interference of electroactive compounds and substrates. Glucose, sucrose and lactose content of the model and food samples were measured with both the improved system and commercial enzyme kits. There was high correlation between the results. This gives us the opportunity to use the improved electrode system for determination of glucose, sucrose and lactose in food samples. Also this result was found to be very promising in extending the spectrum of detectable analytes simply by addition of new columns containing immobilised enzymes that are specific to target analytes.

机译：改进了与包含固定化酶的填充床柱反应器集成的安培酶电极，以仅一次采样即可测量食品样品中的葡萄糖，蔗糖和乳糖含量。在线性，灵敏度和响应时间的基础上研究了系统的性能参数。发现这些值对于葡萄糖为20mm，24.9nA / mM和20s。 20毫米，12.0 nA / mM，蔗糖3分钟;乳糖分别为12 mm，10.8 nA / mM和4分钟。在不进行预处理以及不会干扰电活性化合物和底物的情况下，一次采样对三种分析物的总分析时间少于8分钟。使用改进的系统试剂盒和商业酶试剂盒测量模型和食品样品中的葡萄糖，蔗糖和乳糖含量。结果之间具有高度相关性。这使我们有机会使用改进的电极系统测定食品样品中的葡萄糖，蔗糖和乳糖。同样，发现该结果在增加可检测的分析物的光谱方面非常有希望，只需添加含有对目标分析物具有特异性的固定化酶的新色谱柱即可。

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《Nahrung》 |2002年第3期|共5页
作者
Boyaci IH; Mutlu M;
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正文语种 eng
中图分类食品工业;
关键词
Enzyme-immobilised column reactor; Enzyme electrode; Packed-bed column; Biosensors; Milk;

机译：酶固定柱反应器;酶电极;填充床柱;生物传感器;牛奶;

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1. Measurement of glucose, sucrose and lactose in food samples with enzyme-immobilised packed-bed column reactors integrated to an amperometric enzyme electrode [J] . Boyaci IH, Mutlu M Nahrung . 2002,第3期

机译：使用固定在安培酶电极上的酶固定式填充柱反应器测量食品中的葡萄糖，蔗糖和乳糖
2. Amperometric flow-injection determination of sucrose with a mediated tri-enzyme electrode based on sucrose phosphorylase and electrocatalytic oxidation of NADH [J] . Maestre E., Dominguez E., Katakis I. Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2001,第1a2期

机译：蔗糖磷酸化酶和NADH的电催化氧化介导的三酶电极安培流式测定蔗糖
3. Hydrolysis of Lactose by #beta#-Glycosidase CelB from Hyperthermophilic Archaeon Pyrococcus furiosus (Comparison of Hollow-Fiber Membrane and Packed-Bed Immobilized Enzyme Reactors for Continuous Processing of Ultrahigh Temperature-Treated Skim Milk) [J] . Barbara Splechtna, Inge Petzelbauer, Bernhard Kuhn, Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology . 2002,第0期

机译：嗜热古生热球菌＃beta＃-糖苷酶CelB水解乳糖（中空纤维膜和填充床固定酶反应器连续处理超高温脱脂牛奶的比较）
4. High-Linearity Glucose Enzyme Electrodes for Food Industres: Preparation by a Plasma Polymerization Technique [C] . Mehmet Mutlu, Selma Mutlu, Ismail H. Boyaci, National meeting of the American Chemical Society . 1998

机译：用于食品工业的高线性葡萄糖酶电极：通过等离子体聚合技术制备
5. Analytical Parameters of an Amperometric Glucose Biosensor for Fast Analysis in Food Samples [O] . Margalida Artigues, Jordi Abellà, Sergi Colominas 2017

机译：安培型葡萄糖生物传感器用于食品样品快速分析的分析参数
6. Combi-CLEAs of Glucose Oxidase and Catalase for Conversion of Glucose to Gluconic Acid Eliminating the Hydrogen Peroxide to Maintain Enzyme Activity in a Bubble Column Reactor [O] . Agnes Mafra, Letícia Ulrich, Jakub Kornecki, 2019

机译：葡萄糖氧化酶的组合和过氧化氢酶，用于将葡萄糖转化为葡萄糖酸，消除过氧化氢以维持泡沫柱反应器中的酶活性
7. Wired Enzyme Electrodes for Amperometric Determination of Glucose or Lactate inthe Presence of Interfering Substances. (Reannouncement with New Availability Information) [R] . Ohara, T. J., Rajajopalan, R., Heller, A. 1994

机译：用于电化学测定葡萄糖或乳酸盐的有线酶电极在干扰物质存在下的应用。（重新公布新的可用性信息）

Measurement of glucose, sucrose and lactose in food samples with enzyme-immobilised packed-bed column reactors integrated to an amperometric enzyme electrode

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