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Self-assembled silk sericin/poloxamer nanoparticles as nanocarriers of hydrophobic and hydrophilic drugs for targeted delivery

机译：自组装的丝胶蛋白/泊洛沙姆纳米颗粒作为疏水性和亲水性药物的纳米载体，用于靶向递送

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In recent times self-assembled micellar nanoparticles have been successfully employed in tissue engineering for targeted drug delivery applications. In this review, silk sericin protein from non-mulberry Antheraea mylitta tropical tasar silk cocoons was blended with pluronic F-127 and F-87 in the presence of solvents to achieve self-assembled micellar nanostructures capable of carrying both hydrophilic (FITC-inulin) and hydrophobic (anticancer drug paclitaxel) drugs. The fabricated nanoparticles were subsequently characterized for their size distribution, drug loading capability, cellular uptake and cytotoxicity. Nanoparticle sizes ranged between 100 and 110 nm in diameter as confirmed by dynamic light scattering. Rapid uptake of these particles into cells was observed in in vitro cellular uptake studies using breast cancer MCF-7 cells. In vitro cytotoxicity assay using paclitaxel-loaded nanoparticles against breast cancer cells showed promising results comparable to free paclitaxel drugs. Drug-encapsulated nanoparticle-induced apoptosis in MCF-7 cells was confirmed by FACS and confocal microscopic studies using Annexin V staining. Up-regulation of pro-apoptotic protein Bax, down-regulation of anti-apoptotic protein Bcl-2 and cleavage of regulatory protein PARP through Western blot analysis suggested further drug-induced apoptosis in cells. This study projects silk sericin protein as an alternative natural biomaterial for fabrication of self-assembled nanoparticles in the presence of poloxamer for successful delivery of both hydrophobic and hydrophilic drugs to target sites.
机译：最近，自组装的胶束纳米颗粒已成功用于组织工程中，用于靶向药物递送应用。在这篇综述中，将非桑蚕Antheraea mylitta热带tasar蚕茧的蚕丝胶蛋白与普朗尼克F-127和F-87在溶剂存在下混合，以实现能够同时携带两种亲水性分子的自组装胶束纳米结构（FITC-菊粉）和疏水性（抗癌药紫杉醇）药物。随后对制备的纳米颗粒的尺寸分布，药物负载能力，细胞摄取和细胞毒性进行了表征。通过动态光散射证实，纳米粒子的直径范围在直径100至110 nm之间。在使用乳腺癌MCF-7细胞的体外细胞吸收研究中，观察到这些颗粒迅速吸收到细胞中。使用载有紫杉醇的纳米粒子对乳腺癌细胞的体外细胞毒性试验显示了与游离紫杉醇药物相当的有希望的结果。通过FACS和使用膜联蛋白V染色的共聚焦显微镜研究证实了药物包裹的纳米颗粒诱导的MCF-7细胞凋亡。凋亡蛋白Bax的上调，抗凋亡蛋白Bcl-2的下调以及通过蛋白质印迹分析对调节蛋白PARP的裂解表明药物进一步诱导了细胞凋亡。这项研究将丝胶蛋白作为可替代的天然生物材料，用于在泊洛沙姆的存在下制造自组装纳米颗粒，以成功地将疏水性和亲水性药物递送至靶位。

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