Antibody-directed targeting of lysostaphin adsorbed onto polylactide nanoparticles increases its antimicrobial activity against S. aureus in vitro

Satishkumar R.; Vertegel A.A.

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Antibody-directed targeting of lysostaphin adsorbed onto polylactide nanoparticles increases its antimicrobial activity against S. aureus in vitro

机译：溶血葡萄球菌素吸附在聚乳酸纳米颗粒上的抗体定向靶向增强了其对金黄色葡萄球菌的体外抗菌活性

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The objective of this paper was to study the effect of antibody-directed targeting of S. aureus by comparing the activities of lysostaphin conjugated to biodegradable polylactide nanoparticles (NPs) in the presence and in the absence of co-immobilized anti-S. aureus antibody. Lysostaphin-antibody-NP conjugates were synthesized through physical adsorption at different enzyme:antibody:NP ratios. The synthesized enzyme-NP conjugates were characterized by means of dynamic light scattering and zeta potential analysis, and the total protein binding yield on the NPs was characterized using Alexa Fluor 350 and 594 dyes for the S. aureus antibody and lysostaphin respectively. We observed enhanced antimicrobial activity for both enzyme-coated and enzyme-antibody-coated NPs for lysostaphin coatings corresponding to ～ 40% of the initial monolayer and higher compared to the free enzyme case (p < 0.05). At the highest antibody coating concentration, bacterial lysis rates for antibody-coated samples were significantly higher than for lysostaphin-coated samples lacking the antibody (p < 0.05). Such enzyme-NP conjugates thus have the potential for becoming novel therapeutic agents for treating antibiotic-resistant S. aureus infections.

机译：本文的目的是通过比较在存在和不存在共同固定化抗S的情况下，缀合至生物可降解聚乳酸纳米颗粒（NPs）的溶葡萄球菌素的活性，研究抗体针对金黄色葡萄球菌的靶向作用。金黄色抗体。通过物理吸附以不同的酶：抗体∶NP比率合成溶葡萄球菌素-抗体-NP缀合物。合成的酶-NP共轭物通过动态光散射和ζ电位分析进行表征，分别使用金黄色葡萄球菌抗体和溶葡萄球菌素的Alexa Fluor 350和594染料表征在NP上的总蛋白结合率。我们观察到溶血磷脂涂层的酶包被和酶抗体包被的NPs的抗菌活性均增强，相当于初始单层的约40％，与游离酶相比更高（p <0.05）。在最高抗体包被浓度下，抗体包被样品的细菌裂解率显着高于缺乏抗体的溶葡萄球菌素包被样品（p <0.05）。因此，这种酶-NP缀合物具有成为治疗抗生素抗性金黄色葡萄球菌感染的新型治疗剂的潜力。

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《Nanotechnology》 |2011年第50期|共10页
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Satishkumar R.; Vertegel A.A.;
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1. Antibody-directed targeting of lysostaphin adsorbed onto polylactide nanoparticles increases its antimicrobial activity against S. aureus in vitro [J] . Satishkumar R., Vertegel A.A. Nanotechnology . 2011,第50期

机译：溶血葡萄球菌素吸附在聚乳酸纳米颗粒上的抗体定向靶向增强了其对金黄色葡萄球菌的体外抗菌活性
2. Ceftaroline pharmacodynamic activity versus community-associated and healthcare-associated methicillin-resistant Staphylococcus aureus, heteroresistant vancomycin-intermediate S. aureus, vancomycin-intermediate S. aureus and vancomycin-resistant S. aureus using an in vitro model. [J] . Zhanel GG, Rossnagel E, Nichol K, The Journal of Antimicrobial Chemotherapy . 2011,第6期

机译：头孢洛林的药效学活性与社区相关和医疗保健相关的耐甲氧西林金黄色葡萄球菌，耐杂万古霉素的中间金黄色葡萄球菌，耐万古霉素的中间金黄色葡萄球菌和耐万古霉素的金黄色葡萄球菌的体外药理活性。
3. In vitro activity of oritavancin against community-associated meticillin-resistant Staphylococcus aureus (CA-MRSA), vancomycin-intermediate S. aureus (VISA), vancomycin-resistant S. aureus (VRSA) and daptomycin-non-susceptible S. aureus (DNSSA) [J] . Saravolatz L.D., Pawlak J., Johnson L.B. International journal of antimicrobial agents . 2010,第1期

机译：奥利万星对社区相关的耐甲氧西林金黄色葡萄球菌（CA-MRSA），万古霉素中间金黄色葡萄球菌（VISA），耐万古霉素的金黄色葡萄球菌（VRSA）和达托霉素非敏感性金黄色葡萄球菌（DNSSA）的体外活性）
4. Antimicrobial Activities of Conventional and Novel Antimicrobial Agents against S. aureus Biofilms [C] . Jason Kang, Bingyun Li Society for Biomaterials annual meeting and exposition . 2018

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5. Properties of silver and copper nanoparticle-containing aqueous solutions and evaluation of their in vitro activity against Candida albicans and Staphylococcus aureus biofilms [D] . Montes Aguirre, Melissa Mariluz 2015

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6. In Vitro Activity of Recombinant Lysostaphin against Staphylococcus aureus Isolates from Anterior Nares and Blood [O] . Christof von Eiff, John F. Kokai-Kun, Karsten Becker, 2003

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机译：2％葡萄糖酸氯己定，1％次氯酸钠和对羟基苯酚与呋喃霉素对抗金黄色葡萄球菌，白色念珠菌，粪肠球菌和金黄色葡萄球菌的抗菌活性= 2％氯己定抗菌活性，1％次氯酸钠和对氯苯酚与呋喃，金黄色葡萄球菌，白色念珠菌，粪肠球菌和金黄色葡萄球菌

Antibody-directed targeting of lysostaphin adsorbed onto polylactide nanoparticles increases its antimicrobial activity against S. aureus in vitro

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