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首页> 外文期刊>Microbes and infection >The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone stimulates phagocytic activity in human macrophages through the p38 MAPK pathway
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The Pseudomonas aeruginosa quorum-sensing molecule N-(3-oxododecanoyl)-L-homoserine lactone stimulates phagocytic activity in human macrophages through the p38 MAPK pathway

机译：铜绿假单胞菌群体感应分子N-（3-氧十二烷酰基）-L-高丝氨酸内酯通过p38 MAPK途径刺激人巨噬细胞的吞噬活性

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摘要

Quorum-sensing is an important mechanism for the regulation of bacteria-to-bacteria communication. Recent advances have demonstrated that the Pseudomonas aeruginosa signaling molecule N-(3-oxododecanoyl)-L-homoserine lactone (3O-C-12-HSL) is also a potent modulator of eukaryotic cells and may thus play an important role in the host response during P. aeruginosa infections. Little is known, however, about specific effects of 3O-C-12-HSL molecules on human macrophages. To address this issue, we investigated the influence of 3O-C-12-HSL on the phagocytic activity, production of reactive oxygen species, and activation of p38 and p42/44 MAPK signaling pathways in human macrophages. We show an effect of 3O-C-12-HSL on the phagocytic capacity in human macrophages, which depends on concentration and time of exposure. When cells were exposed to 100 mu M 3O-C-12-HSL for 30 min or 1 h, the phagocytic activity increased 1.8 and 1.6 times, respectively. The 3O-C-12-HSL treatments had no significant effect on the level of reactive oxygen species production. Furthermore, the p38 MAPK, but not the p42/44 MAPK, signaling pathway was activated in response to 3O-C-12-HSL. In addition, specific blocking of p38 MAPK activation with 10 mu M SB 203580 prevented the 3O-C-12-HSL-induced increase in the phagocytic activity. These findings demonstrate that the bacterial quorum-sensing can play a significant role also in regulation of macrophage activity during infections caused by P. aeruginosa. (C) 2005 Elsevier SAS. All rights reserved.
机译：群体感应是调节细菌与细菌之间交流的重要机制。最近的进展表明，铜绿假单胞菌信号分子N-（3-氧十二烷酰基）-L-高丝氨酸内酯（3O-C-12-HSL）也是真核细胞的有效调节剂，因此可能在宿主反应中起重要作用在铜绿假单胞菌感染期间。然而，关于3O-C-12-HSL分子对人类巨噬细胞的特定作用知之甚少。为了解决这个问题，我们研究了3O-C-12-HSL对人类巨噬细胞中吞噬活性，活性氧产生以及p38和p42 / 44 MAPK信号通路活化的影响。我们显示了3O-C-12-HSL对人类巨噬细胞吞噬能力的影响，这取决于浓度和暴露时间。当细胞暴露于100μM 3O-C-12-HSL 30分钟或1小时时，吞噬活性分别增加了1.8倍和1.6倍。 3O-C-12-HSL处理对活性氧产生水平没有明显影响。此外，响应3O-C-12-HSL，激活了p38 MAPK，但未激活p42 / 44 MAPK信号通路。此外，用10μM SB 203580特异性阻断p38 MAPK激活可防止3O-C-12-HSL诱导的吞噬活性增加。这些发现表明，在由铜绿假单胞菌引起的感染期间，细菌群体感应也可以在巨噬细胞活性的调节中起重要作用。 （C）2005 Elsevier SAS。版权所有。

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