Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells

Rajneesh Malhotra; Malcolm Ward; Helen Bright; Richard Priest; Martyn R. Foster; Michael Hurle; Eddie Blair; Michael Bird

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Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells

机译：上皮细胞上潜在的呼吸道合胞病毒受体的分离和鉴定

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Respiratory syncytial virus (RSV) infection causes severe lower respiratory diseases in infancy, early childhood and the elderly. RSV infections respond poorly to current therapies. Therefore, we initiated a search for novel drug targets by investigating the characteristics and identify of RSV adhesion receptors on mammalian cells. Soluble human lectins, complex polysaccharides and a low molecular selectin antagonist, TBC1269, were used to characterise and isolate the RSV receptor on a human epithelial cell line (Hep2 cells). The binding characteristics of the RSV receptor of Hep2 cells were similar to those reported for L-selectin. The carbohydrate-based selectin antagonists, fucoidan and TBC 1269, inhibit RSV infection both in vitro and in a mouse model of infection. Furthermore, we have isolated anmexin II as a potential RSV receptor on Hep2 cells. The expression of annexin II was increased after RSV infection. Recombinant annexin II binds to RSV G-protein, heparin and plasminogen and the binding is inhibited by a selectin antagonist, TBC1269, These findings in dicate that inhibitors of annexin II could have potential in treating RSV infection.

机译：呼吸道合胞病毒（RSV）感染在婴儿，幼儿和老年人中引起严重的下呼吸道疾病。 RSV感染对当前疗法的反应较差。因此，我们通过研究哺乳动物细胞中RSV粘附受体的特征和鉴定，开始寻找新的药物靶标。可溶性人类凝集素，复合多糖和低分子选择素拮抗剂TBC1269用于表征和分离人类上皮细胞系（Hep2细胞）上的RSV受体。 Hep2细胞的RSV受体的结合特性与L-选择蛋白的结合特性相似。基于碳水化合物的选择素拮抗剂岩藻依聚糖和TBC 1269在体外和小鼠感染模型中均抑制RSV感染。此外，我们已经分离出Anmexin II作为Hep2细胞上的潜在RSV受体。 RSV感染后膜联蛋白II的表达增加。重组膜联蛋白II与RSV G蛋白，肝素和纤溶酶原结合，并且该结合受到选择蛋白拮抗剂TBC1269的抑制。这些发现表明，膜联蛋白II抑制剂可能具有治疗RSV感染的潜力。

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《Microbes and infection》 |2003年第2期|共11页
作者
Rajneesh Malhotra; Malcolm Ward; Helen Bright; Richard Priest; Martyn R. Foster; Michael Hurle; Eddie Blair; Michael Bird;
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正文语种 eng
中图分类细胞生物学;
关键词
annexin II; RSV; G-protein; TBC1269; fucoidan;

机译：膜联蛋白II;RSV;G蛋白;TBC1269;岩藻依丹;

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1. Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells [J] . Rajneesh Malhotra, Malcolm Ward, Helen Bright, Microbes and infection . 2003,第2期

机译：上皮细胞上潜在的呼吸道合胞病毒受体的分离和鉴定
2. Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection [J] . Vira Bitko, Nicolle E Garmon, Tin Cao, BMC Microbiology . 2004,第1期

机译：呼吸道合胞病毒感染的角膜上皮细胞中细胞因子和NF-κB的激活：眼部炎症和呼吸道感染的潜在相关性
3. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. [J] . Arnold R, Neumann M, Konig W Immunology: An Official Journal of the British Society for Immunology . 2007,第1期

机译：过氧化物酶体增殖物激活的受体-γ激动剂抑制呼吸道合胞病毒诱导的人肺上皮细胞中细胞间粘附分子-1的表达。
4. PPARgamma- and Toll-Like Receptor-2 Agonists Might Have Therapeutical Value in Respiratory Syncytial Virus (RSV)-Induced Airway Diseases PPARgamma Agonists May Act in a PPARgamma-Independent Manner [C] . R. Arnold, W. Konig Collegium Internationale Allergologicum . 2007

机译：pParγ-和Toll样受体-2激动剂可能对呼吸道合胞病毒（RSV）的治疗值（RSV）诱导的气道疾病Pparγ激动剂可以以ppargamma独立的方式行事
5. Activation of host cell gene expression by respiratory syncytial virus infection of the alveolar epithelial cell line A549: A role for the redox responsive transcription factor NF-kappaB. [D] . Carpenter, Laura Rosenbalm. 2002

机译：呼吸道合胞病毒感染肺泡上皮细胞系A549激活宿主细胞基因表达：氧化还原反应性转录因子NF-κB的作用。
6. Activation of cytokines and NF-kappa B in corneal epithelial cells infected by respiratory syncytial virus: potential relevance in ocular inflammation and respiratory infection [O] . Vira Bitko, Nicolle E Garmon, Tin Cao, 2004

机译：呼吸道合胞病毒感染的角膜上皮细胞中细胞因子和NF-κB的激活：眼部炎症和呼吸道感染的潜在相关性
7. The Expression of Histamine Receptor and Chemokines in Epithelial Cells and Fibroblasts of the Upper Respiratory Tract after Respiratory Syncytial Virus (RSV) Infection [O] . Su Jung Young, Jung Seang Cho 2011

机译：呼吸合胞病毒（RSV）感染后呼吸道上皮细胞组胺受体和趋化因子的表达及上呼吸道成纤维细胞
8. Respiratory Syncytial Virus Infection of Human Primary Nasal and Bronchial Epithelial Cell Cultures and Bronchoalveolar Macrophages. [R] . Becker, S., Soukup, J., Yankaskas, J. 1992

机译：呼吸道合胞病毒感染人原发性鼻和支气管上皮细胞培养及支气管肺泡巨噬细胞。

Isolation and characterisation of potential respiratory syncytial virus receptor(s) on epithelial cells

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