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Biofunctionalization of CeF3 : Tb3+ nanoparticles

机译:CeF3:Tb3 +纳米粒子的生物功能化

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CeF3: Tb3+ nanoparticles (short pillar-like morphology with an average length and width of 11 and 5 nm, respectively) were successfully prepared by a polyol process using diethyleneglycol (DEG) as solvent. After being functionalized with a SiO2-NH2 layer, these CeF3: Tb3+ nanoparticles can be conjugated with biotin molecules (activated by thionyl chloride) and further with avidin. The as-formed CeF3: Tb3+ nanoparticles, CeF3: Tb3+ nanoparticles functionalized with amino groups, biotin conjugated amino-functionalized CeF3: Tb3+ nanoparticles and biotinylated CeF3: Tb3+ nanoparticles bonded with avidin were characterized by x-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR), UV/vis absorption spectra and luminescence spectra, respectively. The biofunctionalization of the CeF3: Tb3+ nanoparticles has less effect on their luminescence properties, i.e. they still show strong green emission (from Tb3+, with D-5(4) - F-7(5) at 543 nm as the most prominent group), indicative of the great potential for these CeF3: Tb3+ nanoparticles to be used as biological fluorescence probes.
机译:通过使用二甘醇(DEG)作为溶剂的多元醇工艺成功地制备了CeF3:Tb3 +纳米颗粒(分别为短柱状形态,平均长度和宽度分别为11和5 nm)。在用SiO2-NH2层官能化后,这些CeF3:Tb3 +纳米颗粒可以与生物素分子(被亚硫酰氯激活),以及与抗生物素蛋白共轭。通过X射线衍射(XRD),透射电子显微镜对形成的CeF3:Tb3 +纳米颗粒,经氨基官能化的CeF3:Tb3 +纳米颗粒,生物素偶联的氨基官能化CeF3:Tb3 +纳米颗粒以及与生物素结合的生物素化CeF3:Tb3 +纳米颗粒进行了表征。 (TEM),傅立叶变换红外(FT-IR),UV / vis吸收光谱和发光光谱。 CeF3:Tb3 +纳米粒子的生物功能化对其发光特性的影响较小,即它们仍显示出强绿色发射(来自Tb3 +,其中543 nm处的D-5(4)-F-7(5)是最突出的基团) ,表明将这些CeF3:Tb3 +纳米颗粒用作生物荧光探针的巨大潜力。

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