采用水热法制备了上转换发光纳米材料,并对其表面采用改良的St(o)ber法包覆二氧化硅,进一步采用硅烷化试剂进行氨基化修饰,最后通过戊二醛交联将亲和素成功偶联到纳米粒子表面,制备了亲和素生物功能化上转化发光纳米粒子.对制备的纳米粒子及其表面功能化处理过程进行了荧光光谱、TEM,XRD,FT- IR表征.结果表明,功能化后的上转换发光纳米粒子与亲和素成功结合,并保持了良好的上转换发光特性,可应用于生物标记.%Upconversion nanophosphors were prepared by a solvothermal method. Then the surface of the upconversion nanophosphors was capped with silica by a modified Stober method. It was further treated with silane coupling agent to modify amino groups onto the surface of the nanophosphors. Finally, avidin was coupled onto the surface of the nanophosphors using glutaraldehyde as cross-linker. So, a avidin - functionalized upconversion nanophosphors was successfully prepared. The nanophosphors together with its functionalized process were characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), fourier transform infrared (FT-IR) spectroscopy, respectively. The results suggested that the functionalized nanophosphors have successfully conjugated with avidin and still keep its upconversion luminescence property.
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