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首页> 外文期刊>Nanotechnology >Interaction between carbon nanotubes and mammalian cells: characterization by flow cytometry and application
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Interaction between carbon nanotubes and mammalian cells: characterization by flow cytometry and application

机译：碳纳米管与哺乳动物细胞之间的相互作用：通过流式细胞仪表征和应用

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We show herein that CNT-cell complexes are formed in the presence of a magnetic field. The complexes were analyzed by flow cytometry as a quantitative method for monitoring the physical interactions between CNTs and cells. We observed an increase in side scattering signals, where the amplitude was proportional to the amount of CNTs that are associated with cells. Even after the formation of CNT-cell complexes, cell viability was not significantly decreased. The association between CNTs and cells was strong enough to be used for manipulating the complexes and thereby conducting cell separation with magnetic force. In addition, the CNT-cell complexes were also utilized to facilitate electroporation. We observed a time constant from CNT-cell complexes but not from cells alone, indicating a high level of pore formation in cell membranes. Experimentally, we achieved the expression of enhanced green fluorescence protein by using a low electroporation voltage after the formation of CNT-cell complexes. These results suggest that higher transfection efficiency, lower electroporation voltage, and miniaturized setup dimension of electroporation may be accomplished through the CNT strategy outlined herein.
机译：我们在这里表明，CNT-细胞复合物是在磁场的存在下形成的。通过流式细胞仪分析复合物，作为监测CNT与细胞之间物理相互作用的定量方法。我们观察到侧面散射信号的增加，其幅度与与细胞相关的CNT的数量成比例。即使在形成CNT-细胞复合物之后，细胞活力也没有显着降低。 CNT与细胞之间的缔合足够强，可以用于操纵复合物，从而利用磁力进行细胞分离。另外，CNT-细胞复合物也用于促进电穿孔。我们从CNT细胞复合物中观察到时间常数，但从单独的细胞中观察不到，这表明细胞膜中的孔形成水平很高。通过实验，我们在形成CNT细胞复合物后通过使用低电穿孔电压实现了增强的绿色荧光蛋白的表达。这些结果表明，可以通过本文概述的CNT策略来实现更高的转染效率，更低的电穿孔电压和电穿孔的小型化安装尺寸。

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