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A mesoporous silica nanosphere-based drug delivery system using an electrically conducting polymer

机译：使用导电聚合物的基于介孔二氧化硅纳米球的药物递送系统

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摘要

In this study, a mesoporous silica nanoparticle (MSN)-based nerve growth factor (NGF) delivery system has been successfully embedded within an electroactive polypyrrol (Ppy). The spherical particles with similar to 100 nm diameter possess a large surface-to-volume ratio for the entrapment of NGF into the pores of MSNs while retaining their bioactivity. Direct incorporation of MSN-NGF within Ppy was achieved during electrochemical polymerization. The loading amount and release profile of NGF from the composite was investigated by sandwich ELISA. The NGF incorporation can be controllable by varying particle concentration or by extending electrodeposition time. The morphology and chemical composition of the Ppy/MSN-NGF composite was evaluated by atomic force microscopy (AFM), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and x-ray photoelectron spectroscopy (XPS). Optical and electron microscopy revealed a characteristic attachment of PC 12 cells and the outgrowth of their neurites when grown on the Ppy/MSN-NGF composite as a result of a sustained and controlled release of NGF. In order to observe the effectiveness of electrical stimulation, neurite extension of cells cultured on unstimulated and stimulated Ppy/MSN-NGF was compared. The NGF release in the presence of electrical stimulation promoted significantly greater neurite extension.
机译：在这项研究中，基于介孔二氧化硅纳米粒子（MSN）的神经生长因子（NGF）输送系统已成功嵌入电活性聚吡咯（Ppy）中。直径接近100 nm的球形颗粒具有较大的表面体积比，可将NGF截留在MSNs的孔中，同时保留其生物活性。在电化学聚合过程中，将MSN-NGF直接掺入Ppy中。通过夹心ELISA研究了NGF从复合物中的负载量和释放曲线。 NGF的掺入可以通过改变颗粒浓度或延长电沉积时间来控制。通过原子力显微镜（AFM），透射电子显微镜（TEM），扫描电子显微镜（SEM）和X射线光电子能谱（XPS）评估了Ppy / MSN-NGF复合材料的形态和化学组成。光学和电子显微镜显示，由于NGF的持续和受控释放，当在Ppy / MSN-NGF复合材料上生长时，PC 12细胞具有特征性的附着以及其神经突的长出。为了观察电刺激的有效性，比较了在未刺激和刺激的Ppy / MSN-NGF上培养的细胞的神经突延伸。在电刺激的存在下，NGF的释放促进了明显的神经突扩展。

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