• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nanotechnology >A smart multifunctional nanocomposite for intracellular targeted drug delivery and self-release
【24h】

A smart multifunctional nanocomposite for intracellular targeted drug delivery and self-release

机译：用于细胞内靶向药物递送和自我释放的智能多功能纳米复合材料

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A multifunctional 'all-in-one' nanocomposite is fabricated using a colloid, template and surface-modification method. This material encompasses magnetic induced target delivery, cell uptake promotion and controlled drug release in one system. The nanocomposite is characterized by scanning electron microscopy, transmission electron microscopy, x-ray diffraction, N_2 adsorption and vibrating sample magnetometry. The prepared material has a diameter of 350-400nm, a high surface area of 420.29m~2g~(-1), a pore size of 1.91nm and a saturation magnetization of 32emug~(-1). Doxorubicin (DOX) is loaded in mesopores and acid-sensitive blockers are introduced onto the orifices of the mesopores by a Schiff base linker to implement pH-dependent self-release. Folate was also introduced to improve DOX targeted delivery and endocytosis. The linkers remained intact to block pores with ferrocene valves and inhibit the diffusion of DOX at neutral pH. However, in lysosomes of cancer cells, which have a weak acidic pH, hydrolysis of the Schiff base group removes the nanovalves and allows the trapped DOX to be released. These processes are demonstrated by UV-visible absorption spectra, confocal fluorescence microscopy images and methyl thiazolyl tetrazolium assays in vitro, which suggest that the smart nanocomposite successfully integrates targeted drug delivery with internal stimulus induced self-release and is a potentially useful material for nanobiomedicine.
机译：使用胶体，模板和表面修饰方法制造多功能“多合一”纳米复合材料。该材料在一个系统中包含磁性诱导的靶标传递，细胞吸收促进和受控药物释放。纳米复合材料的特征在于扫描电子显微镜，透射电子显微镜，x射线衍射，N_2吸附和振动样品磁力分析。所制备的材料的直径为350-400nm，高表面积为420.29m〜2g〜（-1），孔径为1.91nm，饱和磁化强度为32emug〜（-1）。阿霉素（DOX）装在中孔中，酸敏感的阻滞剂通过Schiff碱接头引入中孔的孔中，以实现pH依赖的自释放。还引入了叶酸以改善DOX靶向递送和内吞作用。接头保持完整，以二茂铁阀阻塞孔并抑制DOX在中性pH下的扩散。但是，在酸性pH值较弱的癌细胞溶酶体中，席夫碱的水解会除去纳米阀，并释放出所捕获的DOX。这些过程通过紫外可见吸收光谱，共聚焦荧光显微镜图像和甲基噻唑基四唑鎓体外测定法得以证明，这表明该智能纳米复合材料成功地将靶向药物递送与内部刺激诱导的自我释放相结合，并且是纳米生物医学的潜在有用材料。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号