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Preparation and characterization of a magnetic and optical dual-modality molecular probe

机译：磁光双模态分子探针的制备与表征

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摘要

Multi-modality imaging probes combine the advantages of individual imaging techniques to yield highly detailed anatomic and molecular information in living organisms. Herein, we report the synthesis and characterization of a dual-modality nanoprobe that couples the magnetic properties of ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) with the near infrared fluorescence of Cy5.5. The fluorophore is encapsulated in a biocompatible shell of silica surrounding the iron oxide core for a final diameter of similar to 17 nm. This silica-coated iron oxide nanoparticle (SCION) has been analyzed by transmission electron microscopy, dynamic light scattering, and superconducting quantum interference device (SQUID). The particle demonstrates a strong negative surface charge and maintains colloidal stability in the physiological pH range. Magnetic hysteresis analysis confirms superparamagnetic properties that could be manipulated for thermotherapy. The viability of primary human monocytes, T cells, and B cells incubated with the particle has been examined in vitro. In vivo analysis of agent leakage into subcutaneous A431 tumors in mice was also conducted. This particle has been designed for diagnostic application with magnetic resonance and fluorescence imaging, and has future potential to serve as a heat-sensitive targeted drug delivery platform.
机译：多模态成像探头结合了单个成像技术的优势，可以在生物体内产生高度详细的解剖和分子信息。在这里，我们报告双模式纳米探针的合成和表征，该探针结合了超小型超顺磁性氧化铁纳米粒子（USPIOs）的磁性与Cy5.5的近红外荧光。荧光团被包裹在氧化铁核周围的生物相容性二氧化硅壳中，最终直径类似于17 nm。已经通过透射电子显微镜，动态光散射和超导量子干涉装置（SQUID）分析了这种二氧化硅包覆的氧化铁纳米粒子（SCION）。该颗粒表现出强的负表面电荷，并在生理pH范围内保持胶体稳定性。磁滞分析证实了可用于热疗的超顺磁特性。已在体外检查了与该粒子孵育的原代人单核细胞，T细胞和B细胞的生存能力。还进行了体内试剂渗入小鼠皮下A431肿瘤的分析。该颗粒已设计用于磁共振和荧光成像的诊断应用，并且有潜力用作热敏靶向药物输送平台。

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