...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature cell biology >DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila.
【24h】

DmPAR-6 directs epithelial polarity and asymmetric cell division of neuroblasts in Drosophila.

机译：DmPAR-6指导果蝇中神经母细胞的上皮极性和不对称细胞分裂。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Drosophila protein Bazooka is required for both apical-basal polarity in epithelial cells and directing asymmetric cell division in neuroblasts. Here we show that the PDZ-domain protein DmPAR-6 cooperates with Bazooka for both of these functions. DmPAR-6 colocalizes with Bazooka at the apical cell cortex of epithelial cells and neuroblasts, and binds to Bazooka in vitro. DmPAR-6 localization requires Bazooka, and mislocalization of Bazooka through overexpression redirects DmPAR-6 to ectopic sites of the cell cortex. In the absence of DmPAR-6, Bazooka fails to localize apically in neuroblasts and epithelial cells, and is distributed in the cytoplasm instead. Epithelial cells lose their apical-basal polarity in DmPAR-6 mutants, asymmetric cell divisions in neuroblasts are misorientated, and the proteins Numb and Miranda do not segregate correctly into the basal daughter cell. Bazooka and DmPAR-6 are Drosophila homologues of proteins that direct asymmetric cell division in early Caenorhabditis elegans embryos, and our results indicate that homologous protein machineries may direct this process in worms and flies.
机译：果蝇蛋白质火箭筒对于上皮细胞的顶基极极性和指导成神经细胞的不对称细胞分裂都是必需的。在这里，我们显示了PDZ结构域蛋白DmPAR-6与火箭筒共同发挥了这两个功能。 DmPAR-6与火箭筒共定位在上皮细胞和神经母细胞的顶细胞皮层，并在体外与火箭筒结合。 DmPAR-6本地化需要火箭筒，而火箭筒通过过表达的错误定位会将DmPAR-6重定向到细胞皮质的异位部位。在没有DmPAR-6的情况下，火箭筒无法在神经母细胞和上皮细胞中顶端定位，而是分布在细胞质中。上皮细胞在DmPAR-6突变体中失去其顶基极性，成神经细胞中的不对称细胞分裂方向错误，蛋白质Numb和Miranda不能正确地分离到基底子细胞中。火箭筒和DmPAR-6是果蝇的同源蛋白，可指导秀丽隐杆线虫早期胚胎中的不对称细胞分裂，我们的结果表明同源蛋白机制可能在蠕虫和果蝇中指导这一过程。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号