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首页> 外文期刊>Natural product communications >Methodology for Porphyrin Isolation by High-Performance Countercurrent Chromatography
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Methodology for Porphyrin Isolation by High-Performance Countercurrent Chromatography

机译：高性能逆流色谱法分离卟啉的方法学

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摘要

Countercurrent chromatography is a versatile technique for the isolation of a wide variety of plant substances. However, little attention has been devoted to the application of this technique for the isolation of porphyrins. This class of compounds are of great importance in the medical area and in photocatalysis due to their heterocyclic structure, composed of four modified pyrrol subunits interconnected on their a carbon atoms by methinic bridges. The methanol extract of Gallesia integrifolia was partitioned using different solvents; the dichloromethane fraction was then submitted to countercurrent chromatography. The solvent system composed of n-hexane, ethyl acetate, methanol, and water (1:2.5:2.5:1) was chosen to perform the chromatographic analysis due to the enhanced solubility and the best distribution coefficients of the target compounds. Two porphyrins were isolated by this method and identified as 132-hydroxypheophorbide a methyl ester and pheophorbide a, methyl ester. The solvent system proposed provided good distribution coefficients for both substances (1.27 and 1.87, respectively), and a high resolution factor.
机译：逆流色谱法是一种用于分离多种植物物质的通用技术。但是，很少有人关注该技术在卟啉分离中的应用。这类化合物由于其杂环结构而在医学领域和光催化领域具有重要意义，它们的杂环结构由四个修饰的吡咯亚基组成，它们的碳原子之间通过甲基桥相互连接。用不同的溶剂分配整叶加仑的甲醇提取液。然后将二氯甲烷级分进行逆流色谱。由于目标化合物具有更高的溶解度和最佳分配系数，因此选择了由正己烷，乙酸乙酯，甲醇和水（1：2.5：2.5：1）组成的溶剂系统以进行色谱分析。通过这种方法分离出两个卟啉，并将其鉴定为132-羟基脱镁叶绿酸一种甲酯和脱镁叶绿酸a，一种甲基酯。提出的溶剂系统为两种物质提供了良好的分配系数（分别为1.27和1.87），并且具有较高的分离度。

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