...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Natural product communications >Dialyzable Leukocyte Extracts Activate TLR-2 on Monocytes
【24h】

Dialyzable Leukocyte Extracts Activate TLR-2 on Monocytes

机译：可透析的白细胞提取物激活单核细胞上的TLR-2

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dialyzable leukocyte extracts (DLE) transfer specific cell-mediated immune responses from sensitized donors to non-immune recipients. In addition, DLE have several immunomodulatory effects and are used for the treatment of several infectious and non-infectious diseases. Previous studies showed that human DLE obtained from virus-infected leukocytes and bovine DLE decrease the production of the pro-inflammatory cytokine TNF-a in response to bacterial lipopolysaccharide, in vitro and in vivo. In the present work, we inquire as to whether DLE from uninfected human leukocytes have the ability to regulate cytokine production in peripheral blood mononuclear cells (PBMC) in vitro. We observed that PBMC from healthy individuals were able to produce TNF-a, IL-12 and IL-10 after stimulation with DLE. Moreover, we identified monocytes as the main cell population that produced TNF-a after DLE stimulation. Interestingly, we found that DLE contain unidentified ligands that activate Toll-like receptor (TLR)-2. Finally, we observed that DLE directly activated monocytes through TLR-2. These results reveal a new biological activity of DLE, and suggest that part of the immunomodulatory properties of DLE could be attributed to TLR-2 activation on monocytes and to the induction ofa pro-inflammatory environment that is crucial for control of infectious diseases.
机译：可透析的白细胞提取物（DLE）将特定的细胞介导的免疫反应从致敏的供体转移到非免疫的受体。此外，DLE具有多种免疫调节作用，可用于治疗多种传染性和非传染性疾病。先前的研究表明，在体外和体内，从病毒感染的白细胞和牛DLE获得的人DLE会降低对细菌脂多糖的促炎性细胞因子TNF-α的产生。在本工作中，我们询问未感染的人类白细胞产生的DLE是否具有调节体外外周血单个核细胞（PBMC）中细胞因子产生的能力。我们观察到健康个体的PBMC在DLE刺激后能够产生TNF-a，IL-12和IL-10。此外，我们确定单核细胞是DLE刺激后产生TNF-α的主要细胞群。有趣的是，我们发现DLE包含激活Toll样受体（TLR）-2的未知配体。最后，我们观察到DLE通过TLR-2直接激活了单核细胞。这些结果揭示了DLE的新生物学活性，并表明DLE的部分免疫调节特性可归因于单核细胞上的TLR-2激活以及诱导对控制传染病至关重要的促炎性环境。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号