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首页> 外文期刊>Natural product communications >Development and Validation of LC-MS/MS Method for Quantitative Determination of Adenosine, Guanosine, Xanthine and Uric acid in Widely Consumed Vegetables in Thailand
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Development and Validation of LC-MS/MS Method for Quantitative Determination of Adenosine, Guanosine, Xanthine and Uric acid in Widely Consumed Vegetables in Thailand

机译：LC-MS / MS法定量测定泰国大吃蔬菜中腺苷，鸟苷，黄嘌呤和尿酸的建立和验证

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In this study, a triple quadrupole LC-MS/MS electrospray ionization method was developed and validated for quantitative determination of adenosine, guanosine, xanthine and uric acid in fifteen widely consumed Thai vegetables. The method was successively developed by using caffeine as internal standard. The lower limit of quantitation (LLOQ) was 0.2 mu g/g for adenosine and guanosine, and 1.0 mu g/g for uric acid and xanthine. The method was fully validated according to USFDA guidelines and all performance characteristics were found acceptable. Subsequently, the developed and validated LC-MS/MS method was applied to determine the four interest substances in fifteen widely consumed vegetables in Thailand. The results showed that all vegetables included in the study could be classified as low adenosine, guanosine, xanthine and uric acid containing foods since the concentrations of these substances were less than 50 mg per 100 g. This finding was enormously valuable information for hyperuricemia and gouty patients.
机译：在这项研究中，开发了一种三重四极杆LC-MS / MS电喷雾电离方法，并经过验证可用于定量测定15种广泛食用的泰国蔬菜中的腺苷，鸟苷，黄嘌呤和尿酸。以咖啡因为内标相继开发了该方法。腺苷和鸟苷的定量下限（LLOQ）为0.2μg / g，尿酸和黄嘌呤的定量下限为1.0μg / g。该方法已根据USFDA指南进行了充分验证，所有性能特征均可接受。随后，使用经过开发和验证的LC-MS / MS方法确定了泰国15种广泛消费的蔬菜中的4种重要物质。结果表明，该研究中包括的所有蔬菜都可以归为含低腺苷，鸟嘌呤，黄嘌呤和尿酸的食物，因为这些物质的浓度低于每100克50毫克。这一发现对于高尿酸血症和痛风患者是非常有价值的信息。

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