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Antifungal and insecticidal activity of two Juniperus essential oils

机译:两种Jun草精油的抗真菌和杀虫活性

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Essential oils of two Tibetan Junipers Juniperus saltuaria and J. squamata var. fargesii (Cupressaceae) were obtained by distilling dried leaves and branches using a Clevenger apparatus. Sixty-seven compounds from J. saltuaria and 58 from J. squamata var. fargesii were identified by gas chromatography-mass spectrometry (GC-MS). Both essential oils contained similar ratios of four abundant monoterpenoids: 44 and 35% sabinene, 13 and 9% elemol, 8 and 7% terpinen-4-ol, and 4 and 17% α-pinene, respectively. These oils had antifungal activity based on a direct bioautography assay of Colletotrichum acutatum, C. fragariae, and C. gloeosporioides, and insecticidal activity based on serial-time mortality bioassay of azalea lace bugs, Stephanitis pyrioides. Antifungal activity of Juniperus oils was weak when compared with commercial fungicides such as benomyl and captan. Whole Juniperus oils at quarter the dosage used against Colletotrichum species were more insecticidal than 10 mg/mL malathion, killing ≥70 - 90% adult lace bugs after 4 hours of exposure. R_f values of 0.18 for J. saltuaria oil and 0.19 for J. squamata oil indicated lipophilic monoterpenes which were the putative sources of biological activity.
机译:两种藏式杜松杜鹃和盐生J. squamata var的精油。通过使用Clevenger装置蒸馏干燥的叶子和树枝可得到fargesii(柏科)。盐生J.saltuaria的67种化合物和鳞茎J. squamata var。的58种化合物。通过气相色谱-质谱法(GC-MS)鉴定了fargesii。两种精油都包含四种丰富的单萜类化合物的相似比率:分别为44%和35%的sa烯,13%和9%的elemol,8%和7%的萜品烯-4-醇以及4%和17%的α-pine烯。这些油具有基于切菜炭疽菌,草莓角斑病菌和球形孢子虫的直接生物自显影测定法的抗真菌活性,以及​​基于杜鹃花臭虫(Stephanitis pyrioides)的连续时间死亡率生物测定法的杀虫活性。与商业杀菌剂(如苯菌灵和克菌丹)相比,杜松油的抗真菌活性较弱。相对于10 mg / mL马拉硫磷,使用四分之一的全朱古力油对炭疽菌的杀虫力更高,接触4小时后杀死≥70-90%的成虫。 R.f值对于盐生菜链霉菌油为0.18,对于S. squamata油菜籽油为0.19,表明亲脂性单萜是生物活性的推定来源。

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