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首页> 外文期刊>Natural product communications >Morphological, Chemical and Molecular Characterization of Centella asiatica Germplasms for Commercial Cultivation in the Indo-Gangetic Plains
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Morphological, Chemical and Molecular Characterization of Centella asiatica Germplasms for Commercial Cultivation in the Indo-Gangetic Plains

机译:在印度恒河平原商业化种植的积雪积雪草种质的形态,化学和分子特征

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摘要

Centella asiatica germplasm collected from north, north-eastern and southern parts of India was compared for biomass and centellosides productivity under uniform agro-climatic conditions of the Indo-Gangetic plains at Lucknow. The highest biomass accumulation (411.9 g FW/m~2 area) was recorded in accession A from north India, followed by 284.0, 135.7 and 29.2 g FW/m~2 in accessions M, В and E from southern, eastern and north-eastern regions, respectively. Accession M possessed the highest asiaticoside content (52.1 mg/gDW) that was 1.58, 2.34 and 21.7 folds more than accessions А, В and E, respectively. The madecassoside level in leaves of accessions В and M was comparable (28.9 and 25.7 mg/gDW) and two folds more than accession A (13.9 mg/gDW). The madecassic and asiatic acid content in leaf tissue of all four accessions remained low in Lucknow. Amplified fragment length polymorphism (AFLP) analysis with 23 primers yielded 696 fragments, 563 of which were polymorphic. Accession M out-grouped with genetic dissimilarity indices of 83, 85 and 95% from accessions A, E and B, respectively. Commercial cultivation of accessions M and A through a four months growth cycle (June to September) in agro-climatic conditions of the Indo-Gangetic plains is suggested.
机译:比较了印度北部,东北部和南部地区积雪的积雪草种质,在勒克瑙的印度恒河平原农业气候条件一致的条件下,其生物量和百脑香苷的生产力。在印度北部,A品种的生物量积累最高(411.9 g FW / m〜2面积),随后在南部,东部和北部,M,В和E品种的生物量最高,分别为284.0、135.7和29.2 g FW / m〜2。东部地区。品种M的积雪草苷含量最高(52.1 mg / gDW),分别比品种А,В和E高1.58、2.34和21.7倍。零件号В和M的叶子中的马卡德甙水平相当(28.9和25.7 mg / gDW),是零件号A(13.9 mg / gDW)的两倍。在勒克瑙,所有四个种质的叶片组织中的腐殖酸和积雪草酸含量仍然较低。用23个引物进行的扩增片段长度多态性(AFLP)分析产生696个片段,其中563个为多态性。种质M与A,E和B种分别具有83%,85%和95%的遗传相似性指数。建议在印度-恒河平原的农业气候条件下,通过四个月的生长周期(6月至9月)对种质M和A进行商业化种植。

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