Antigen-induced translocation of PKC-θ to membrane rafts is required for T cell activation

Kun Bi; Yoshihiko Tanaka; Nolwenn Coudronniere; Katsuji Sugie; Sooji Hong; Marianne J. B. van Stipdonk; Amnon Altman

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Antigen-induced translocation of PKC-θ to membrane rafts is required for T cell activation

机译：T细胞活化需要抗原诱导的PKC-θ易位至膜筏

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Protein kinase C-θ (PKC-θ) is essential for mature T cell activation; however, the mechanism by which it is recruited to the TCR signaling machinery is unknown. Here we show that T cell stimulation by antibodies or peptide-major histocompatibility complex (MHC) induces translocation of PKC-θ to membrane lipid rafts, which localize to the immunological synapse. Raft translocation was mediated by the PKC-θ regulatory domain and required Lck but not ZAP-70. In addition, PKC-θ was associated with Lck in the rafts. An isolated PKC-θ catalytic fragment did not partition into rafts or activate the transcription factor NF-κB, although addition of a Lck-derived raft-localization sequence restored these functions. Thus, physiological T cell activation translocates PKC-θ to rafts, which localize to the T cell synapse; this PKC-θ translocation is important for its function.

机译：蛋白激酶C-θ（PKC-θ）对于成熟的T细胞激活至关重要。但是，将其募集到TCR信令机制的机制尚不清楚。在这里我们显示抗体或肽-主要组织相容性复合物（MHC）刺激T细胞诱导PKC-θ易位到膜脂质筏，其定位于免疫突触。筏移位由PKC-θ调节域介导，需要Lck，但不需要ZAP-70。另外，PKC-θ与筏中的Lck有关。分离的PKC-θ催化片段没有分配到筏中，也没有激活转录因子NF-κB，尽管添加了Lck衍生的筏定位序列可以恢复这些功能。因此，生理性T细胞活化将PKC-θ移位至筏，筏位于T细胞突触。 PKC-θ易位对其功能很重要。

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《Nature immunology》 |2001年第6期|共8页
作者
Kun Bi; Yoshihiko Tanaka; Nolwenn Coudronniere; Katsuji Sugie; Sooji Hong; Marianne J. B. van Stipdonk; Amnon Altman;
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正文语种 eng
中图分类基础医学;
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1. Antigen-induced translocation of PKC-θ to membrane rafts is required for T cell activation [J] . Kun Bi, Yoshihiko Tanaka, Nolwenn Coudronniere, Nature immunology . 2001,第6期

机译：T细胞活化需要抗原诱导的PKC-θ易位至膜筏
2. EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain. [J] . Venkateswarlu K, Gunn Moore-F, Tavare JM, Journal of Cell Science . 1999,第Pta12期

机译：EGF和NGF刺激的细胞粘附素1向PC12细胞质膜的移位需要PI 3激酶激活和功能性的细胞粘附素1 PH域。
3. Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology d [J] . Venkateswarlu K, Gunn-Moore F, Oatey PB, The Biochemical Journal . 1998,第Pta1期

机译：神经生长因子和表皮生长因子刺激的ADP-核糖基化因子交换因子GRP1易位至PC12细胞质膜需要激活磷脂酰肌醇3-激酶和GRP1 pleckstrin同源性d
4. TRANSLOCATION OF MACROMOLECULES ACROSS MEMBRANES AND THROUGH AQUEOUS CHANNELS: Translocation across membranes [C] . Sanford M. Simonauthor_name/, NATO NATO advanced research workshop on biological, biophysical theoretical aspects of polymer structure and transport . 2002

机译：穿过膜的大分子易刻和通过含水通道：跨膜易位
5. Graft antigen-specific CD4+ T cells require CD154 expression to clonally expand and differentiate to the TH1 phenotype and initiate mTOR dependant intimal hyperplasia in cardiac allografts. [D] . Huddleston, Stephen James. 2009

机译：嫁接抗原特异性CD4 + T细胞需要CD154表达才能克隆扩增并分化为TH1表型，并在心脏同种异体移植中引发mTOR依赖性内膜增生。
6. Activation of integrin α5 mediated by flow requires its translocation to membrane lipid rafts in vascular endothelial cells [O] . Xiaoli Sun, Yi Fu, Mingxia Gu, 2016

机译：通过流动介导的整联蛋白α5的激活需要将其易位至血管内皮细胞的膜脂质筏
7. DOCK2 Is Essential for Antigen-Induced Translocation of TCR and Lipid Rafts, but Not PKC-θ and LFA-1, in T Cells [O] . Sanui Terukazu, Inayoshi Ayumi, Noda Mayuko, 2003

机译：DOCK2对于T细胞中抗原诱导的TCR和脂质筏的转运是必需的，但对于PKC-θ和LFA-1则不是

Antigen-induced translocation of PKC-θ to membrane rafts is required for T cell activation

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