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首页> 外文期刊>Mycotoxin Research >Detection and discrimination between ochratoxin producer and non-producer strains of Penicillium nordicum on a ham-based medium using an electronic nose.
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Detection and discrimination between ochratoxin producer and non-producer strains of Penicillium nordicum on a ham-based medium using an electronic nose.

机译：使用电子鼻在基于火腿的培养基上检测和区分曲霉生产者和非生产者 Nondicum 菌株。

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摘要

The aim of this work was to evaluate the potential use of qualitative volatile patterns produced by Penicillium nordicum to discriminate between ochratoxin A (OTA) producers and non-producer strains on a ham-based medium. Experiments were carried out on a 3% ham medium at two water activities (aw ; 0.995, 0.95) inoculated with P. nordicum spores and incubated at 25 degrees C for up to 14 days. Growing colonies were sampled after 1, 2, 3, 7 and 14 days, placed in 30-ml vials, sealed and the head space analysed using a hybrid sensor electronic nose device. The effect of environmental conditions on growth and OTA production was evaluated based on the qualitative response. However, after 7 days, it was possible to discriminate between strains grown at 0.995 aw, and after 14 days, the OTA producer and non-producer strain and the controls could be discriminated at both aw levels. This study suggests that volatile patterns produced by P. nordicum strains may differ and be used to predict the presence of toxigenic contaminants in ham. This approach could be utilised in ham production as part of a quality assurance system for preventing OTA contamination.
机译：这项工作的目的是评估潜在利用北青霉（Penicillium nordicum）产生的定性挥发性模式来区分基于火腿的培养基上OT曲霉毒素A（OTA）生产者和非生产者菌株的区别。实验是在3％的火腿培养基上以 P接种的两个水活度（ a w ; 0.995，0.95）进行的。北欧孢子，并在25摄氏度下温育长达14天。在1、2、3、7和14天后对生长的菌落进行采样，放入30 ml的小瓶中，密封，并使用混合传感器电子鼻装置分析顶空。根据定性反应评估了环境条件对生长和OTA产生的影响。但是，在7天后，可以区分以0.995 a w 生长的菌株，而14天后，OTA生产者和非生产者菌株以及对照可以在两个 w 级别上进行区分。这项研究表明由 P产生的挥发性模式。北欧菌株可能有所不同，可用于预测火腿中的产毒污染物。此方法可在火腿生产中用作防止OTA污染的质量保证体系的一部分。

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