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首页> 外文期刊>Biological Control: Theory and Application in Pest Management >Combined application of botanical formulations and biocontrol agents for the management of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt in banana
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Combined application of botanical formulations and biocontrol agents for the management of Fusarium oxysporum f. sp. cubense (Foc) causing Fusarium wilt in banana

机译：植物制剂和生物防治剂联合应用治疗尖孢镰刀菌f。 sp。引起香蕉枯萎病的立方体病（Foc）

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摘要

Plant products along with biocontrol agents were tested against Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense (Foc). Of the 22 plant species tested, the leaf extract of Datura metel (10%) showed complete inhibition of the mycelial growth of Foc. Two botanical fungicides, Wanis 20 EC and Damet 50 EC along with selected PGPR strains with known biocontrol activity, Pseudomonas fluorescens 1, Pf1 and Bacillus subtilis, TRC 54 were tested individually and in combination for the management of Fusarium wilt under greenhouse and field conditions. Combined application of botanical formulation and biocontrol agents (Wanis 20 EC + Pf1 + TRC 54) reduced the wilt incidence significantly under greenhouse (64%) and field conditions (75%). Reduction in disease incidence was positively correlated with the induction of defense-related enzymes peroxidase (PO) and polyphenol oxidase (PPO). Three antifungal compounds (two glycosides and one ester) in D. metel were separated and identified using TLC, RP-HPLC (Reverse Phase-High Pressure Liquid Chromatography) and mass spectrometry. In this study it is clear that combined application of botanical formulations and biocontrol agents can be very effective in the management of Fusarium wilt of banana.
机译：对植物产品和生物防治剂进行了抗尖孢镰刀菌f引起的香蕉枯萎病的测试。 sp。立方（Foc）。在测试的22种植物中，曼陀罗的叶提取物（占10％）显示出对Foc菌丝体生长的完全抑制。分别测试了两种植物杀菌剂Wanis 20 EC和Damet 50 EC以及选定的具有已知生物防治活性的PGPR菌株荧光假单胞菌1，Pf1和枯草芽孢杆菌TRC 54，并联合测试了它们在温室和田间条件下对枯萎病的管理。植物制剂和生物防治剂（Wanis 20 EC + Pf1 + TRC 54）的联合应用显着降低了温室（64％）和田间条件（75％）的枯萎率。疾病发病率的降低与防御相关酶过氧化物酶（PO）和多酚氧化酶（PPO）的诱导呈正相关。分离并使用TLC，RP-HPLC（反相高压液相色谱）和质谱法鉴定和鉴定了梅氏螺旋体中的三种抗真菌化合物（两种糖苷和一种酯）。在这项研究中，很明显，将植物制剂与生物防治剂联合使用可对香蕉枯萎病的防治非常有效。

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