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首页> 外文期刊>Natural product research >Antioxidant and DNA damage protective properties of anthocyanin-rich extracts from Hibiscus and Ocimum: A comparative study
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Antioxidant and DNA damage protective properties of anthocyanin-rich extracts from Hibiscus and Ocimum: A comparative study

机译：芙蓉和罗汉果中富含花青素的提取物的抗氧化和DNA损伤保护特性：对比研究

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摘要

Anthocyanin extracts (AEs) from Ocimum tenuiflorum (leaf), Hibiscus rosa-sinensis (petal) and Hibiscus sabdariffa (calyx) were investigated and compared for in vitro antioxidant activity and DNA damage protective property. Total phenolic content (TPC) and total anthocyanin content (TAC) of the AEs were determined and the major anthocyanins were characterised. In vitro antioxidant activities were assessed by ferric-reducing antioxidant power (FRAP) assay, 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical-scavenging activity, 2-deoxy-d-ribose degradation assay and lipid peroxidation assay. The protective property of the AEs was also examined against oxidative DNA damage by H2O2 and UV using pUC19 plasmid. All the AEs particularly those from O. tenuiflorum demonstrated efficient antioxidant activity and protected DNA from damage. Strong correlation between antioxidant capacity and TPC and TAC was observed. Significant correlation between antioxidant capacity and TPC and TAC ascertained that phenolics and anthocyanins were the major contributors of antioxidant activity.
机译：研究了罗勒叶（Ocimum tenuiflorum）（叶），芙蓉（Hibiscus rosa-sinensis）（花瓣）和芙蓉（Shibidariffa）（花萼）的花青素提取物（AEs）的体外抗氧化活性和DNA损伤保护性能。测定了AE的总酚含量（TPC）和总花色苷含量（TAC），并表征了主要花色苷。体外抗氧化活性通过三价铁还原抗氧化能力（FRAP）测定，2,2-二苯基-1-苦基肼基（DPPH）自由基清除活性，2-脱氧-d-核糖降解测定和脂质过氧化测定进行评估。还使用pUC19质粒检查了AEs的保护特性，以防止H2O2和UV对DNA的氧化性DNA损伤。所有AE，特别是来自O. tenuiflorum的AE均显示出有效的抗氧化活性，并保护了DNA免受破坏。观察到抗氧化剂能力与TPC和TAC之间有很强的相关性。抗氧化剂能力与TPC和TAC之间的显着相关性确定酚和花色苷是抗氧化剂活性的主要贡献者。

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