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首页> 外文期刊>Natural product research >A novel strategy for rapid quantification of 20(S)-protopanaxatriol and 20(S)-protopanaxadiol saponins in Panax notoginsengP. ginseng and P. quinquefolium
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A novel strategy for rapid quantification of 20(S)-protopanaxatriol and 20(S)-protopanaxadiol saponins in Panax notoginsengP. ginseng and P. quinquefolium

机译：一种快速定量三七中20（S）-原人参三醇和20（S）-原人参二醇皂苷的新颖策略。人参和西洋参

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摘要

A novel strategy for the qualitative and quantitative determination of 20(S)-protopanaxatriol saponins (PTS) and 20(S)-protopanaxadiol saponins (PDS) in Panax notoginseng, Panax ginseng and Panax quinquefolium, based on the overlapping peaks of main components of PTS (calibrated by ginsenoside Rg1) and PDS (calibrated by ginsenoside Rb1), was proposed. The analysis was performed by using high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD). Under specific chromatographic conditions, all samples showed two overlapping peaks containing several main ginsenosides belonging to PTS and PDS, respectively. The overlapping peaks were also identified by using HPLC-MS. Based on the sum and ratio of PTS and PDS, 60 tested Panax samples were divided into three main clusters according to their species. The findings suggested that this strategy provides a simple and rapid approach to quantify PTS and PDS in Panax herbs.
机译：一种基于三七主要成分重叠峰定性和定量测定三七，人参和西洋参中20（S）-原人参三醇皂苷（PTS）和20（S）-原人参二醇酯（PDS）的新策略提出了PTS（由人参皂苷Rg1校准）和PDS（由人参皂苷Rb1校准）。通过使用高效液相色谱结合蒸发光散射检测（HPLC-ELSD）进行分析。在特定的色谱条件下，所有样品均显示两个重叠的峰，分别包含几种分别属于PTS和PDS的主要人参皂苷。重叠的峰也通过HPLC-MS鉴定。根据PTS和PDS的总和和比例，将60个经测试的人参样品根据其种类分为三个主要簇。研究结果表明，该策略提供了一种简单快速的方法来定量三七药中的PTS和PDS。

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