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首页> 外文期刊>Neoplasma: Journal of Experimental and Clinical Oncology >Effect of justicidin B - a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines.
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Effect of justicidin B - a potent cytotoxic and pro-apoptotic arylnaphtalene lignan on human breast cancer-derived cell lines.

机译：Justicidin B-一种有效的细胞毒性和促凋亡芳基萘木脂素对人乳腺癌衍生细胞系的作用。

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摘要

Justicidin B produced by genetically transformed cultures of Linum leonii was tested for cytotoxic activity and induction of apoptosis in MDA-MB-231 and MCF-7 breast cancer derived cell lines. The tested lignan evoked strong, concentration dependent cytotoxicity in both cell lines, whereby MCF-7 proved to be far more sensitive as compared to MDA-MB-231. The 24 h treatment of both cell lines increased the level of apoptotic DNA fragmentation; however the proapoptotic activity is completely inhibited if the cells are co-incubated with the non-selective pan-caspase inhibitor Boc-Asp(OMe)-fluoromethyl ketone (PCI), which implies that justicidin B, activates programmed cell death via caspase -dependent mechanisms. Exposure of MDA-MB-231 cells with justicidin B leads to concentration dependent decrease in the expression of NFkB; whereas the treatment of MCF-7, is consistent with strong increase in the expression of this transcription factor. Keywords: Justicidin B, cytotoxicity, apoptosis, NFkB, MCF-7, MDA-MB-231.
机译：测试了利纳木（Linum leonii）的遗传转化培养物产生的Justicidin B在MDA-MB-231和MCF-7乳腺癌衍生细胞系中的细胞毒活性和诱导的凋亡。测试的木脂素在两种细胞系中均引起强烈的，浓度依赖性的细胞毒性，因此与MDA-MB-231相比，MCF-7的敏感性更高。两种细胞系的24小时处理均会增加凋亡DNA片段化的水平;但是，如果将细胞与非选择性泛胱天蛋白酶抑制剂Boc-Asp（OMe）-氟甲基酮（PCI）共同孵育，则促凋亡活性会被完全抑制，这表明Justicidin B通过caspase依赖性激活程序性细胞死亡机制。用justicidin B暴露MDA-MB-231细胞会导致浓度依赖性的NFkB表达下降;而对MCF-7的治疗与该转录因子表达的强烈增加是一致的。关键字：Justicidin B，细胞毒性，凋亡，NFkB，MCF-7，MDA-MB-231。

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