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首页> 外文期刊>Molecules and cells >Expression analysis of the pistil genes in controlling self-incompatibility of Brassica campestris by CO2 gas using microarray
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Expression analysis of the pistil genes in controlling self-incompatibility of Brassica campestris by CO2 gas using microarray

机译:基因芯片表达雌蕊基因控制CO2气体控制甘蓝型油菜自交不亲和性的表达分析

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In Brassica campestris, self-incompatibility (SI) can be overcome by CO2 gas treatment. Previously, we reported inhibition of the SI in the Brassica campestris line, 734, by CO2 gas. In this study, we used a cDNA microarray comprising 1,184 unique cDNA ESTs from a pistil-specific cDNA library of the Brassica line to examine the expression of the pistil genes in response to CO2. Microarray analysis revealed that expression of 2% of the pistil genes was altered by exposure to CO2 gas. Surprisingly, the SLG gene of the susceptible line was one of the down-regulated genes. Transmission electron microscopic analysis showed that the CO2 caused morphological changes in the papillary cell. Our results demonstrate that the overcoming of SI by CO2 gas involves reduced expression of the SLG gene in Brassica.
机译:在甘蓝型油菜中,可以通过二氧化碳气体处理来克服自身不相容性(SI)。以前,我们报道了CO2气体对甘蓝油菜线734中SI的抑制作用。在这项研究中,我们使用了一个基因芯片,该基因芯片包含来自芸苔属植物雌蕊特异性cDNA库的1,184个独特cDNA EST,以检查雌蕊基因对CO2的表达。芯片分析表明,暴露于二氧化碳气体会改变2%的雌蕊基因的表达。令人惊讶地,易感品系的SLG基因是下调基因之一。透射电镜分析表明,CO2引起了乳头状细胞的形态变化。我们的结果表明,CO2气体克服SI涉及减少甘蓝型油菜SLG基因的表达。

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