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首页> 外文期刊>Nematologia Mediterranea >Effect of pokeweed (Phytolacca americana L.) extract on hatching of Globodera rostochiensis and Meloidogyne spp.
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Effect of pokeweed (Phytolacca americana L.) extract on hatching of Globodera rostochiensis and Meloidogyne spp.

机译:商陆(Phytolacca americana L.)提取物对罗氏沼虾和Meloidogyne spp孵化的影响。

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摘要

The effect of pokeweed (P. americana) fruit extract on hatching of the potato cyst nematode, G. rostochiensis, and the British root-knot nematode, M. artiellia, was studied in Italy under laboratory conditions at 20+or-1 degrees C. Cysts of G. rostochiensis were exposed to a series of increasing pokeweed fruit extract aliquots of 0.01, 0.02, 0.05, 0.1, 0.2, 0.4, 0.6, 0.8 and 1.0 ml, which were added to 3 ml of sodium metavanadate 0.6 mM. Egg masses of M. artiellia were exposed to the same series of increasing fruit extract aliquots that were instead added to 3 ml of water. The controls were the hatching agent sodium metavanadate 0.6 mM, pokeweed root leachate and an aliquot of 1 ml of pokeweed fruit extract added to 3 ml of water for G. rostochiensis, and distilled water only for M. artiellia. The control treatment sodium metavanadate induced 20.7% hatching of G. rostochiensis, whereas the other two controls, pokeweed root leachate and 1 ml fruit extract in 3 ml of water, did not induce any hatching. The hatching of G. rostochiensis was not enhanced by adding to sodium metavanadate aliquots of 0.01-0.05 ml of pokeweed fruit extract, but was significantly increased by adding larger aliquots of 0.1-0.8 ml and suppressed (7.2%) by 1 ml. The greatest hatch increase of G. rostochiensis (81.6%) was provided by adding an aliquot of 0.4 ml of pokeweed fruit extract to 3 ml of sodium metavanadate. The hatching of M. artiellia was 80.5% in the control distilled water alone and was not enhanced by adding 0.01-0.2 ml of pokeweed fruit extract. However, water enrichment with increasing aliquots of 0.4-1 ml fruit extract significantly suppressed (17.5-37.7%) M. artiellia hatching compared to the control. Pokeweed was a very good host for M. incognita and M. javanica and a non-host for M. artiellia..
机译:在意大利的实验室条件下,在20或-1摄氏度的条件下,研究了商陆(P. americana)水果提取物对马铃薯孢囊线虫G. rostochiensis和英国根结线虫M. artiellia的孵化的影响。将G. rostochiensis的囊肿暴露于一系列0.01、0.02、0.05、0.1、0.2、0.4、0.6、0.8和1.0 ml的递增的商陆果实提取物等分试样中,然后将这些等分试样添加到3 ml的偏钒酸钠0.6 mM中。将动脉分枝杆菌的卵团暴露于相同系列的增加的水果提取物等分试样中,取而代之的是将其加入3毫升水中。对照是孵化剂偏钒酸钠0.6 mM,商陆根浸出液和等分的1 ml商陆果实提取物,加到3 ml的罗氏沼虾水中,仅蒸馏水用于大叶念珠菌。对照处理偏钒酸钠诱导了罗氏沼虾的20.7%孵化,而其他两个对照(商陆根浸出液和3 ml水中的1 ml水果提取物)未引起任何孵化。通过添加0.01-0.05 ml商陆果实提取物的偏钒酸钠等分试样,不会增强罗氏沼虾的孵化,但是通过添加0.1-0.8 ml的较大等分试样,显着增加了罗氏沼虾的孵化,但抑制了(7.2%)1 ml。将0.4 ml商陆水果提取物等分试样加到3 ml偏钒酸钠中,可最大程度地增加罗氏沼虾的孵化率(81.6%)。仅在对照蒸馏水中,M。artiellia的孵化率为80.5%,而未通过添加0.01-0.2 ml的商陆果实提取物来增强。然而,与对照相比,增加等分试样的0.4-1 ml水果提取物的富集水可显着抑制(17.5-37.7%)动脉分枝杆菌孵化。商陆是隐孢子虫和爪哇分枝杆菌的非常好的寄主,而对青蒿却不是寄主。

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