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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Cat proenkephalin-A does not contain the opioid octapeptide.
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Cat proenkephalin-A does not contain the opioid octapeptide.

机译：猫前脑啡肽-A不包含阿片类八肽。

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摘要

The sequence of a large cDNA fragment of proenkephalin-A from the cat adrenal medulla was obtained using reverse transcription followed by polymerase chain reaction, and cloning. This cDNA encompasses the region normally containing all the opioid peptides, except the C-terminal heptapeptide. As with other species, cat proenkephalin-A contains four conserved copies of (Met5)-enkephalin, and one of (Leu5)-enkephalin, flanked by processing sites of paired basic amino acids. However, significant differences were found in the nucleotide and deduced amino acid sequences in the region of the octapeptide. In particular, the essential tyrosyl residue is substituted by a histidyl residue, making it unlikely that the cat equivalent would have opioid activity. Furthermore, the peptide is not flanked by paired basic residues, suggesting it is not processed.
机译：使用逆转录随后进行聚合酶链反应和克隆获得了来自猫肾上腺髓质的前脑啡肽-A的大cDNA片段的序列。该cDNA涵盖通常包含除C末端七肽之外的所有阿片样物质肽的区域。与其他物种一样，猫原脑啡肽-A包含（Met5）-脑啡肽的四个保守拷贝，和（Leu5）-脑啡肽的一个拷贝，两侧为成对的碱性氨基酸加工位点。但是，在八肽区域的核苷酸和推导的氨基酸序列中发现了显着差异。特别地，必需的酪氨酰基残基被组氨酸残基取代，使得猫等同物不太可能具有阿片样活性。此外，该肽的侧面没有成对的碱性残基，表明它没有被加工。

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