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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Changes in proteasome activity following transient ischemia.
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Changes in proteasome activity following transient ischemia.

机译：短暂性脑缺血后蛋白酶体活性的变化。

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摘要

Succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide (Suc-LLVY-MCA) hydrolyzing activities of the 20S and 26S proteasomes in the gerbil cortex following transient forebrain ischemia were examined. Using extraction solutions without ATP, only 20S proteasome activity was noted after separation with glycerol gradient centrifugation. When these extracts were incubated with ATP and an ATP-regenerating system prior to glycerol gradient separation, both 20S and 26S proteasome activities were detected. Following 10 min of ischemia, the activity of the 26S proteasomes decreased, whereas the 20S proteasome activity increased after 30 min of reperfusion. These changes returned to the control level after 1 h. The active 26S proteasomes were formed with ATP-dependent association with the 20S proteasomes and several subunits and the 26S proteasomes degraded ubiquitin-protein conjugates. These results indicate that proteasome activity might not be irreversibly impaired after transient ischemia. However, transient inhibition of ATP-dependent conversion of 20S to 26S proteasomes in vitro must be one of the causes of the accumulation of the ubiquitin-protein conjugates in the early reperfusion period.
机译：研究了短暂前脑缺血后沙鼠皮层20S和26S蛋白酶体的琥珀酰-Leu-Leu-Val-Tyr-4-甲基香豆基-7-酰胺（Suc-LLVY-MCA）的水解活性。使用不含ATP的提取液，用甘油梯度离心分离后仅观察到20S蛋白酶体活性。在甘油梯度分离之前，将这些提取物与ATP和ATP再生系统一起孵育时，会同时检测到20S和26S蛋白酶体活性。缺血10分钟后，再灌注30分钟后26S蛋白酶体的活性下降，而20S蛋白酶体活性增加。这些变化在1小时后恢复到控制水平。活性的26S蛋白酶体与20S蛋白酶体和几个亚基具有ATP依赖性结合，并且26S蛋白酶体降解了泛素-蛋白结合物。这些结果表明，短暂性缺血后蛋白酶体活性可能不会不可逆转地受损。然而，体外20S到26S蛋白酶体的ATP依赖性转化的瞬时抑制必须是早期再灌注期间泛素-蛋白结合物积累的原因之一。

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