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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Sulfite leads to neuron loss in the hippocampus of both normal and SOX-deficient rats
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Sulfite leads to neuron loss in the hippocampus of both normal and SOX-deficient rats

机译：亚硫酸盐导致正常和SOX缺乏大鼠海马神经元丢失

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摘要

Sulfites are compounds commonly used as preservatives in foods, beverages and pharmaceuticals. Sulfite is also endogenously generated during the metabolism of sulfur-containing amino acids and drugs. It has been shown that sulfite is a highly toxic molecule. Many studies have examined the effects of sulfite toxicity, but the effect of ingested sulfite on the number of neurons in the hippocampus has not yet been reported. The present study was undertaken to investigate the effect of ingested sulfite on pyramidal neurons by counting cells in CA1 and CA3-2 subdivisions of the rat hippocampus. For this purpose, rats were assigned to one of four groups (6 rats per group): control (C), sulfite (S), deficient (D) and deficient + sulfite (DS). Sulfite oxidase deficiency was established by feeding rats a low molybdenum diet and adding 200 ppm tungsten (W) to their drinking water. Sulfite (70 mg/kg) was also administered to the animals via their drinking water. At the end of the experimental period, the rats were sacrificed by exsanguination under anesthesia, and their brains and livers quickly removed. The livers were used for a SOX activity assay, and the brains were used for neuronal counts in a known fraction of the CA1 and CA3-2 subdivisions of the left hippocampus using the optical fractionator method, which is a stereological method. The results showed that sulfite treatment caused a significant decrease in the total number of pyramidal neurons in three subdivisions of the hippocampus (CA1 and CA3-2) in the S, D and DS groups compared with the control group. It is concluded that exogenous administration of sulfite causes loss of pyramidal neurons in CA1 and CA3-2 subdivisions in both normal and SOX deficient rat hippocampus. This finding provides supporting evidence that sulfite is a neurotoxic molecule.
机译：亚硫酸盐是通常在食品，饮料和药物中用作防腐剂的化合物。亚硫酸盐也是在含硫氨基酸和药物代谢过程中内生产生的。已经显示出亚硫酸盐是高毒性分子。许多研究已经检查了亚硫酸盐毒性的影响，但尚未报道摄入亚硫酸盐对海马神经元数量的影响。本研究旨在通过计算大鼠海马CA1和CA3-2细分中的细胞来研究摄入的亚硫酸盐对锥体神经元的影响。为此，将大鼠分为四组之一（每组6只大鼠）：对照组（C），亚硫酸盐（S），缺陷（D）和缺陷+亚硫酸盐（DS）。通过给大鼠饲喂低钼饮食并向其饮用水中添加200 ppm钨（W），可以建立亚硫酸盐氧化酶缺乏症。还通过动物的饮用水向动物施用亚硫酸盐（70 mg / kg）。实验期结束时，在麻醉下放血处死大鼠，并迅速摘除它们的大脑和肝脏。使用光学分馏器方法，这是一种立体方法，将肝脏用于SOX活性测定，将大脑用于左海马CA1和CA3-2细分的已知部分的神经元计数。结果表明，与对照组相比，亚硫酸盐处理导致S，D和DS组海马三个细分区域（CA1和CA3-2）的锥体神经元总数显着减少。结论是，在正常和SOX缺乏的大鼠海马中，外源性施用亚硫酸盐会导致CA1和CA3-2分区锥体神经元的丢失。这一发现提供了支持性证据，证明亚硫酸盐是一种神经毒性分子。

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