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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Acetyl- and butyrylcholinesterase molecular forms in normal and streptozotocin-diabetic rat retinal pigment epithelium.
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Acetyl- and butyrylcholinesterase molecular forms in normal and streptozotocin-diabetic rat retinal pigment epithelium.

机译：正常和链脲佐菌素-糖尿病大鼠视网膜色素上皮中的乙酰基和丁酰胆碱酯酶分子形式。

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摘要

We studied the composition of molecular forms of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) in normal and streptozotocin-induced diabetic rat retinal pigment epithelium (RPE). Tissues were sequentially extracted with saline (S(1)) and saline-detergent buffers (S(2)). About a 50% decrease in AChE molecular forms was observed in the diabetic RPE compared to the controls. Approximately 70% of the BChE activity in normal RPE was brought into solution and evenly distributed in S(1) and S(2). Analysis of the fractions from RPE revealed the presence of G(A)(1), G(A)(4) and a small proportion of G(H)(4) BChE forms in S(1); whereas G(A)(4) and G(A)(1) molecules predominate in S(2). A 40% decrease in the activity of G(A)(4) in S(2) was observed in the diabetic RPE. Our results show that diabetes caused a remarkable decrease in the activity of cholinesterases molecular forms in the RPE. This might be related to the alterations observed in diabetic retinopathy.
机译：我们研究了正常和链脲佐菌素诱导的糖尿病大鼠视网膜色素上皮细胞（RPE）中乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）分子形式的组成。依次用盐水（S（1））和盐水洗涤剂（S（2））提取组织。与对照相比，在糖尿病RPE中观察到AChE分子形式减少约50％。正常RPE中大约70％的BChE活性被溶解并均匀分布在S（1）和S（2）中。 RPE馏分的分析显示，S（1）中存在G（A）（1），G（A）（4）和一小部分G（H）（4）BChE形式;而G（A）（4）和G（A）（1）分子在S（2）中占主导地位。在糖尿病RPE中观察到S（2）中G（A）（4）的活性降低了40％。我们的结果表明，糖尿病引起RPE中胆碱酯酶分子形式活性的显着下降。这可能与在糖尿病性视网膜病中观察到的改变有关。

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