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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Expression of carbonic anhydrases II, IV, VII, VIII and XII in rat brain after kainic acid induced status epilepticus.
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Expression of carbonic anhydrases II, IV, VII, VIII and XII in rat brain after kainic acid induced status epilepticus.

机译：海藻酸诱导癫痫持续状态后，大鼠大脑中碳酸酐酶II，IV，VII，VIII和XII的表达。

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摘要

Carbonic anhydrases (CAs) are important enzymes in the central nervous system (CNS), where they participate in regulating cerebrospinal fluid (CSF) secretion, blood-brain barrier and glial cell function. Using RT-PCR we found CA XII mRNA in rat and mouse brain. Cloning of rat CA XII revealed 94% homology with the mouse CA XII. To map the putative functional roles of different CAs, we studied the expression and localization of CA II, CA IV, CA VII, CA-related protein (CA-RP) VIII and CA XII mRNAs in rat brain after kainic acid induced epileptic seizures using Northern blot analysis and in situ hybridization. The expression of CA IV, CA VII and CA-RP VIII was somewhat similar: they were expressed in the cortex, hippocampus and midbrain structures and their expression did not change after the kainic acid treatment. The expression of CA II was concentrated in the white matter structures, which is in line with the preferential expression of CA II in the oligodendrocytes. High levels of CA II mRNA were also detected in the choroid plexus. Surprisingly, CA II was induced 3-12 h after seizures in the vulnerable CA1 region. CA XII was expressed in dentate granule cells, cortex and choroid plexus. Kainic acid stimulated CA XII expression throughout the cortical layer I. The observed hippocampal induction of CA II may indicate a pro-apoptotic and/or epileptogenic role of CA II after prolonged seizures. The physiological significance of the observed cortical induction of CA XII remains obscure. Cytosolic CA II is known to participate in CSF secretion, and the high expression of CA XII in the choroid plexus suggests an analogous role for this membrane-bound isozyme.
机译：碳酸酐酶（CAs）是中枢神经系统（CNS）中的重要酶，它们参与调节脑脊液（CSF）的分泌，血脑屏障和神经胶质细胞功能。使用RT-PCR，我们在大鼠和小鼠的大脑中发现了CA XII mRNA。大鼠CA XII的克隆显示与小鼠CA XII有94％的同源性。为了绘制不同CA的假定功能，我们研究了海藻酸诱导癫痫性癫痫发作后大鼠脑中CA II，CA IV，CA VII，CA相关蛋白（CA-RP）VIII和CA XII mRNA的表达和定位。 Northern印迹分析和原位杂交。 CA IV，CA VII和CA-RP VIII的表达有些相似：它们在皮层，海马和中脑结构中表达，并且在海藻酸处理后它们的表达没有变化。 CA II的表达集中在白质结构中，这与CA II在少突胶质细胞中的优先表达相一致。在脉络丛中也检测到高水平的CA II mRNA。出人意料的是，在脆弱的CA1区癫痫发作后3-12小时诱发了CA II。 CA XII在齿状颗粒细胞，皮质和脉络丛中表达。海藻酸刺激整个皮质层I中CA XII的表达。观察到的海马CA II诱导可能表明长时间癫痫发作后CA II具有促凋亡和/或促癫痫作用。观察到的CA XII皮质诱导的生理意义仍然不清楚。已知胞质CA II参与CSF分泌，CA XII在脉络丛中的高表达表明该膜结合同工酶具有类似的作用。

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