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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study
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Involvement of ATP in noxious stimulus-evoked release of glutamate in rat medullary dorsal horn: A microdialysis study

机译：ATP参与大鼠延髓背角有害刺激引起的谷氨酸释放：微透析研究

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摘要

Our electrophysiological studies have shown that both purinergic and glutamatergic receptors are involved in central sensitization of nociceptive neurons in the medullary dorsal horn (MDH). Here we assessed the effects of intrathecal administration of apyrase (a nucleotide degrading enzyme of endogenous adenosine 5-triphosphate [ATP]), a combination of apyrase and 1,3-dipropyl-8-cyclopentylxanthine (DPCPX, an adenosine A1 receptor antagonist), or 2,3-O-2,4,6-trinitrophenyl-adenosine triphosphate (TNP-ATP, a P2X1, P2X3, P2X2/3 receptor antagonist) on the release of glutamate in the rat MDH evoked by application of mustard oil (MO) to the molar tooth pulp. In vivo microdialysis was used to dialyse the MDH every 5 min, and included 3 basal samples, 6 samples after drug treatment and 12 samples following application of MO. Tooth pulp application of MO induced a significant increase in glutamate release in the MDH. Superfusion of apyrase or TNP-ATP alone significantly reduced the MO-induced glutamate release in the MDH, as compared to vehicle. Furthermore, the suppressive effects of apyrase on glutamate release were reduced by combining it with DPCPX. This study demonstrates that application of an inflammatory irritant to the tooth pulp induces glutamate release in the rat MDH in vivo that may be reduced by processes involving endogenous ATP and adenosine.
机译：我们的电生理研究表明，嘌呤能受体和谷氨酸能受体均参与髓质背角（MDH）伤害性神经元的中枢敏化。在这里，我们评估了鞘内注射腺苷三磷酸酶（一种内源性5-三磷酸腺苷[ATP]的核苷酸降解酶），腺苷三磷酸酶和1,3-二丙基-8-环戊基黄嘌呤（DPCPX，腺苷A1受体拮抗剂）的组合的效果，或2，3-O-2，4，6-三硝基苯基腺苷三磷酸（TNP-ATP，一种P2X1，P2X3，P2X2 / 3受体拮抗剂）对芥末油（MO）诱发的大鼠MDH中谷氨酸释放的影响）至磨牙牙髓。体内微透析用于每5分钟透析MDH，包括3个基础样品，药物处理后的6个样品和MO施用后的12个样品。 MO的牙髓应用导致MDH中谷氨酸盐释放的显着增加。与媒介物相比，单独的腺苷三磷酸双磷酸酶或TNP-ATP的超融合显着降低了MO诱导的MDH中MDH释放的谷氨酸盐。此外，将腺苷三磷酸双磷酸酶与DPCPX组合使用可降低其对谷氨酸释放的抑制作用。这项研究表明，向牙髓施加炎症刺激剂可在大鼠MDH中体内诱导谷氨酸释放，该过程可通过涉及内源性ATP和腺苷的过程来减少。

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