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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Analgesic effect of electroacupuncture on chronic neuropathic pain mediated by P2X 3 receptors in rat dorsal root ganglion neurons
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Analgesic effect of electroacupuncture on chronic neuropathic pain mediated by P2X 3 receptors in rat dorsal root ganglion neurons

机译：电针对P2X 3受体介导的大鼠背根神经节神经元慢性神经痛的镇痛作用。

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摘要

Adenosine 5′-triphosphate disodium (ATP) gated P2X receptors, especially the subtype P2X 3, play a key role in transmission of pain signals in neuropathic pain, ATP has been documented to play a significant role in the progression of pain signals, suggesting that control of these pathways through electroacupuncture (EA) is potentially an effective treatment for chronic neuropathic pain. EA has been accepted to effectively manage chronic pain by applying the stimulating current to acupoints through acupuncture needles. To determine the significance of EA on neuropathic pain mediated by P2X 3 receptors in the dorsal root ganglion (DRG) neurons, mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were recorded, and the expression of P2X 3 receptors in the DRG neurons was assessed by immunohistochemistry (IHC) and in situ hybridization (ISH). In addition, the currents which were evoked in DRG neurons isolated from rats following chronic constriction injury (CCI) by the P2X 3 receptors agonists i.e. ATP and α,β-methylen-ATP (α,β-meATP) were examined through the experimental use of whole cell patch clamp recording. The present study demonstrates that EA treatment can increase the MWT and TWL values and decrease the expression of P2X 3 receptors in DRG neurons in CCI rats. Simultaneously, EA treatment attenuates the ATP and α,β-meATP evoked currents. EA may be expected to induce an apparent induce analgesic effect by decreasing expression and inhibiting P2X 3 receptors in DRG neurons of CCI rats. There is a similar effect on analgesic effect between rats with contralateral EA and those with ipsilateral EA.
机译：腺苷5'-三磷酸二钠（ATP）门控的P2X受体，尤其是P2X 3亚型，在神经性疼痛的疼痛信号传递中起关键作用。通过电针（EA）控制这些途径可能是治疗慢性神经性疼痛的有效方法。通过针刺针将刺激电流施加到穴位，EA已被公认为有效地治疗慢性疼痛。为了确定EA对背根神经节（DRG）神经元中P2X 3受体介导的神经性疼痛的重要性，记录了机械撤离阈值（MWT）和热撤退潜伏期（TWL），并在DRG中记录了P2X 3受体的表达。通过免疫组织化学（IHC）和原位杂交（ISH）评估神经元。此外，通过实验用途检查了由P2X 3受体激动剂即ATP和α，β-亚甲基-ATP（α，β-meATP）引起的慢性收缩损伤（CCI）从大鼠分离的DRG神经元中引起的电流。全细胞膜片钳记录。本研究表明，EA处理可以增加CCI大鼠DRG神经元的MWT和TWL值，并降低P2X 3受体的表达。同时，EA处理会减弱ATP和α，β-meATP诱发的电流。通过降低CCI大鼠DRG神经元的表达并抑制P2X 3受体，可以预期EA会诱导明显的镇痛作用。对侧EA大鼠与同侧EA大鼠之间的镇痛作用相似。

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