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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >The effect of hydrogen peroxide upon beta-adrenoceptor density and function in C6 rat glioma cells.
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The effect of hydrogen peroxide upon beta-adrenoceptor density and function in C6 rat glioma cells.

机译：过氧化氢对C6大鼠神经胶质瘤细胞中β肾上腺素受体密度和功能的影响。

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摘要

Hydrogen peroxide has been suggested to play an important role in the pathogenesis of Alzheimer's disease. In the present study, the effects of hydrogen peroxide upon the functional integrity of beta-adrenoceptors have been investigated in C6 glioma cells. Treatment of cells for 24 h with hydrogen peroxide in serum-free medium produced a concentration-dependent cell toxicity, seen both using cell counting and LDH release into medium as end point. There were no large nor consistent changes in either the density of cell surface beta 1, or beta 2-adrenoceptors, measured using the hydrophilic ligand [3H](-)-CGP 12177, nor in either basal, forskolin and isoprenaline-stimulated cAMP responses, following hydrogen peroxide treatment. It is concluded that the decreased adenylyl cyclase activity and responsiveness to Gs stimulation found in post-mortem brain samples from Alzheimer's disease autopsy cases is unlikely to be mediated by hydrogen peroxide.
机译：已经建议过氧化氢在阿尔茨海默氏病的发病机理中起重要作用。在本研究中，已经在C6胶质瘤细胞中研究了过氧化氢对β-肾上腺素受体功能完整性的影响。使用过氧化氢在无血清培养基中处理细胞24小时，产生了浓度依赖性的细胞毒性，使用细胞计数和LDH释放到培养基中作为终点。使用亲水性配体[3H]（-）-CGP 12177测得的细胞表面β1或β2肾上腺素受体的密度没有大的变化或一致的变化，在基础，福斯高林和异丙肾上腺素刺激的cAMP反应中也没有变化，经过过氧化氢处理。结论是，在阿尔茨海默氏病尸检病例的死后脑样本中发现的腺苷酸环化酶活性降低和对Gs刺激的反应性不太可能由过氧化氢介导。

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