...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Changes in proteasome expression and activity during differentiation of neuronal precursor NTera 2 clone D1 cells.
【24h】

Changes in proteasome expression and activity during differentiation of neuronal precursor NTera 2 clone D1 cells.

机译：在神经元前体NTera 2克隆D1细胞分化过程中蛋白酶体表达和活性的变化。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The effect of differentiation of the human neuronal progenitor cell line NTera 2 clone D1 (NT2/D1) by retinoic acid on components of the proteasome system was studied. The chymotrypsin-like and peptidylglutamyl peptide bond hydrolyzing activities of the proteasome increased five weeks after retinoic acid, and following treatment with mitotic inhibitors returned to levels detected in non-differentiated cells. A selective induction of the MHC class II region encoded LMP7 and LMP2 proteasome subunits occurred during differentiation, whereas there were no changes in the expression of the constitutive LMP2 counterpart (delta-subunit) or the constitutive C2 subunit. Immunofluorescence revealed marked LMP7 accumulation in fully differentiated cells, with no changes in the labeling pattern of the constitutive proteasome antigens. The expression of the alpha-subunit of the PA28 proteasome activator was down-regulated in fully differentiated neurons, but was not correlated with changes in enzymatic activity. Changes in proteasome activity and composition may contribute to the processes leading to differentiation of human neurons in vitro and to the properties of fully differentiated neurons.
机译：研究了视黄酸对人神经元祖细胞NTera 2克隆D1（NT2 / D1）分化对蛋白酶体系统组分的影响。视黄酸后五周，蛋白酶体的胰凝乳蛋白酶样肽和肽基谷氨酰胺肽键水解活性增加，用有丝分裂抑制剂处理后恢复到未分化细胞中检测到的水平。 MHC II类区域编码的LMP7和LMP2蛋白酶体亚基的选择性诱导在分化过程中发生，而组成性LMP2对应物（δ亚基）或组成性C2亚基的表达没有变化。免疫荧光显示，LMP7在完全分化的细胞中有明显的积累，而组成型蛋白酶体抗原的标记模式没有变化。在完全分化的神经元中，PA28蛋白酶体激活剂的α亚基的表达下调，但与酶活性的变化无关。蛋白酶体活性和组成的变化可能有助于导致人类神经元体外分化的过程，也可能导致完全分化的神经元的特性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号