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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Effects of ethanol on three endogenous membrane conductances present in Xenopus laevis oocytes.
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Effects of ethanol on three endogenous membrane conductances present in Xenopus laevis oocytes.

机译：乙醇对非洲爪蟾卵母细胞中三种内源膜电导的影响。

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摘要

The Xenopus oocyte expression and recording system has allowed a detailed analysis of the physiology and pharmacology of neuronal ion channels including their sensitivity to ethanol. It is important however, to ascertain the effects of a particular drug on the channels inherently expressed by oocytes to ensure that drug effects ascribed to the expressed recombinant receptors are manifested solely through those receptors. In this study, the effects of ethanol were determined on three endogenous currents that can be elicited in oocytes and other cells by various manipulations. The inward cation current, IC, was activated by perfusing naive oocytes with a divalent-free recording solution. Ethanol (25-100 mM) modestly inhibited IC with 100 mM ethanol producing a 7-8% inhibition of steady state currents. The store-operated or capacitative calcium current (I(SOC)) was activated in thapsigargin-treated oocytes by switching from a calcium-free solution to one containing 10 mM calcium. In thapsigargin-treated oocytes also injected with EGTA to block calcium-activated chloride currents, ethanol (100 mM) had no effect on the store-operated calcium current. In contrast, ethanol (10-100 mM) dose-dependently inhibited the calcium-dependent chloride current (I(Cl(Ca)) in thapsigargin-treated oocytes. A voltage-jump protocol was used to separate the two components of I(Cl(Ca)), I(Cl-1) and I(Cl-2). Under these conditions, ethanol (100 mM) inhibited I(Cl-1) currents to a greater extent (38%) than it did I(Cl-2) currents (14%). These results show that Xenopus oocytes express endogenous ion channels that are differentially sensitive to ethanol.
机译：非洲爪蟾卵母细胞表达和记录系统已对神经元离子通道的生理和药理学（包括其对乙醇的敏感性）进行了详细分析。然而，重要的是确定特定药物对卵母细胞固有表达的通道的作用，以确保归因于表达的重组受体的药物作用仅通过那些受体表现出来。在这项研究中，确定了乙醇对三种内源性电流的影响，这些电流可以通过各种操作在卵母细胞和其他细胞中引发。通过将天然卵母细胞与无二价记录溶液灌注来激活内向阳离子电流IC。乙醇（25-100 mM）与100 mM乙醇适度抑制IC，产生7-8％的稳态电流抑制。通过毒胡萝卜素处理的卵母细胞，通过从无钙溶液切换到含10 mM钙的溶液，激活了存储操作性或电容性钙电流（I（SOC））。在也经thapsigargin处理的卵母细胞中，也注射了EGTA以阻断钙激活的氯离子流，乙醇（100 mM）对贮存操作的钙电流没有影响。相比之下，乙醇（10-100 mM）剂量依赖性地抑制thapsigargin处理的卵母细胞中钙依赖性氯离子电流（I（Cl（Ca））。使用电压跳跃协议来分离I（Cl （Ca）），I（Cl-1）和I（Cl-2）在这种条件下，乙醇（100 mM）比I（Cl -2）电流（14％）。这些结果表明，非洲爪蟾卵母细胞表达对乙醇具有不同敏感性的内源性离子通道。

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