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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Transcription of estrogen receptor alpha and beta in mouse cerebral cortex: effect of age, sex, 17beta-estradiol and testosterone.
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Transcription of estrogen receptor alpha and beta in mouse cerebral cortex: effect of age, sex, 17beta-estradiol and testosterone.

机译：小鼠大脑皮质中雌激素受体α和β的转录：年龄，性别，17β-雌二醇和睾丸激素的影响。

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摘要

Estrogen actions are mainly mediated by estrogen receptor (ER)alpha and ERbeta which in turn are regulated by several factors including age, sex and gonadal steroid hormones 17beta-estradiol and testosterone. In the present study, we have used nuclear run-off assay to examine the effect of these factors on the rate of transcription of ERalpha and ERbeta of mouse cerebral cortex. The run-off assay result was further corroborated with the measurement of steady state level of ERalpha and ERbeta mRNA by semiquantitative RT-PCR method. Our results reveal that ERalpha transcription rate decreases in old mice of both sexes, whereas ERbeta transcription rate decreases only in old females when compared to their adult counterparts. 17beta-Estradiol supplementation reduces the transcription rate of ERalpha and ERbeta in all groups except in adult male while testosterone treatment down regulates the transcription rate of ERalpha and ERbeta in all groups. The semiquantitative RT-PCR analysis reveals that the level of ERalpha mRNA decreases in old male but shows no effect in old female as compared to adult counterpart. In contrast, ERbeta transcript level decreases in old mice of both sexes. Furthermore, ERalpha mRNA level is higher in adult female than in adult male but no sex-dependent difference is seen in ERbeta mRNA level. Supplementation of 17beta-estradiol shows no significant alteration but testosterone reduces the ERalpha level in male mice, while 17beta-estradiol and testosterone down regulate the ERalpha level in female mice of both ages. In case of ERbeta, 17beta-estradiol decreases the transcript level in all groups except adult male while testosterone treatment results in the down regulation of transcript level in all groups. Thus these findings suggest differential effects of age, sex, 17beta-estradiol and testosterone supplementation on the transcription of mouse ER genes which may account for differences in the protein levels of ERalpha and ERbeta and their functions in the brain.
机译：雌激素的作用主要是由雌激素受体（ER）α和ERbeta介导的，而这些因子又受年龄，性别和性腺类固醇激素17β-雌二醇和睾丸激素等几个因子的调节。在本研究中，我们已使用核径流测定法检查了这些因素对小鼠大脑皮层ERalpha和ERbeta转录速率的影响。通过半定量RT-PCR方法测定ERalpha和ERbeta mRNA的稳态水平，进一步证实了径流测定结果。我们的结果表明，与成年雌性相比，雌雄同体的老龄小鼠中的ERalpha转录速率降低，而雌成年小鼠中的ERbeta转录速率仅降低。除成年男性外，17β-雌二醇补充剂可降低所有组中ERalpha和ERbeta的转录率，而睾丸激素治疗可下调所有组中ERalpha和ERbeta的转录率。半定量RT-PCR分析显示，与成年男性相比，老年男性中ERalpha mRNA的水平降低，但老年女性中无影响。相反，雌雄同体的老龄小鼠中ERbeta转录水平降低。此外，成年女性的ERalpha mRNA水平高于成年男性，但ERbeta mRNA水平未见性别依赖性差异。补充17β-雌二醇无明显变化，但睾丸激素可降低雄性小鼠的ERalpha水平，而17β-雌二醇和睾丸激素可下调两个年龄雌性小鼠的ERalpha水平。在ERbeta的情况下，除成年男性外，所有组中的17beta-雌二醇均会降低转录水平，而睾丸激素治疗会导致所有组中的转录水平下调。因此，这些发现表明年龄，性别，17β-雌二醇和睾丸酮补充剂对小鼠ER基因转录的不同影响，这可能解释了ERalpha和ERbeta的蛋白质水平及其在大脑中的功能的差异。

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