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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Effects of swim stress on mRNA and protein levels of steroid 5alpha-reductase isozymes in prefrontal cortex of adult male rats.
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Effects of swim stress on mRNA and protein levels of steroid 5alpha-reductase isozymes in prefrontal cortex of adult male rats.

机译：游泳压力对成年雄性大鼠前额叶皮质类固醇5α-还原酶同工酶mRNA和蛋白水平的影响。

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摘要

The metabolite of progesterone, allopregnanolone, is among the most potent known ligands of the gamma-aminobutyric acid receptor complex (GABA(A)-R) in the central nervous system. This neuroactive steroid is markedly increased in an animal model of acute stress. Allopregnanolone is synthesized from progesterone by steroidogenic enzymes 5alpha-reductase (5alpha-R) and 3alpha-hydroxysteroid dehydrogenase (3alpha-HSD), with the former being the rate-limiting enzyme in this reaction sequence. In this paper, a quantitative RT-PCR method coupled to laser-induced fluorescence capillary electrophoresis (LIF-CE) and Western blot were used to measure both mRNA and protein levels of 5alpha-R type 1 (5alpha-R1) and 5alpha-R type 2 (5alpha-R2) isozymes in prefrontal cortex of male rats after acute swim stress situations. Our results demonstrate that both 5alpha-R isozymes are significantly higher in prefrontal cortex of male rats after acute swim stress in comparison with control rats. These data may open up a new research line that could improve our understanding of the role of 5alpha-R isozymes in processes that accompany stress situations.
机译：孕酮的代谢物，异戊烷醇，是中枢神经系统中最有力的已知γ-氨基丁酸受体复合物（GABA（A）-R）的配体之一。在急性应激的动物模型中，这种神经活性类固醇明显增加。异戊烷醇酮是通过类固醇生成酶5α-还原酶（5alpha-R）和3α-羟基类固醇脱氢酶（3alpha-HSD）从孕酮合成的，前者是该反应序列中的限速酶。在本文中，采用定量RT-PCR方法结合激光诱导的荧光毛细管电泳（LIF-CE）和Western印迹来测量5alpha-R 1型（5alpha-R1）和5alpha-R的mRNA和蛋白质水平急性游泳应激后雄性大鼠前额叶皮层的2型（5alpha-R2）同工酶我们的研究结果表明，与对照组相比，急性游泳应激后雄性大鼠前额叶皮层的5α-R同工酶均显着较高。这些数据可能会开辟一条新的研究方向，从而可以增进我们对5alpha-R同工酶在伴随压力情况的过程中的作用的了解。

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