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首页> 外文期刊>Biological & pharmaceutical bulletin >Simultaneous Determination of the Lactone and Carboxylate Forms of 7-Ethyl-10-hydroxycamptothecin (SN-38), the Active Metabolite of Irinotecan (CPT-11), in Rat Plasma by High Performance Liquid Chromatography
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Simultaneous Determination of the Lactone and Carboxylate Forms of 7-Ethyl-10-hydroxycamptothecin (SN-38), the Active Metabolite of Irinotecan (CPT-11), in Rat Plasma by High Performance Liquid Chromatography

机译：高效液相色谱法同时测定大鼠血浆中7-乙基-10-羟基喜树碱（SN-38）（伊立替康（CPT-11）的活性代谢物）的内酯和羧酸盐形式

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摘要

We established a high performance liquid chromatography (HPLC) method for the simultaneous determination of the lactone and carboxylate forms of 7-ethyl-10-hydroxycamptothecin (SN-38), the active metabolite of the antitumor drug irinotecan (CPT-11), in rat plasma. Plasma samples were pretreated with chilled MeOH and zinc sulfate to precipitate protein, and were then directly injected into the HPLC system. Chromatography was carried out with a Puresil C18 column (particle size 5/im), and the mobile phase consisted of 0.1m ammonium acetate buffer (pH 5.5) and acetonitrile (70/30, v/v) containing 20 dim of tetra-w-pentylammonium bromide. The column effluent was monitored with a spectrofluorometer (excitation wavelength 380 nm, emission wavelength 540 nm). The method was valid for SN-38 lactone (5—2500 ng/ml) and carboxylate (5—1000 ng/ml).
机译：我们建立了一种高效液相色谱（HPLC）方法，同时测定抗肿瘤药伊立替康（CPT-11）的活性代谢物7-乙基-10-羟基喜树碱（SN-38）的内酯和羧酸盐形式。大鼠血浆。血浆样品用冷却的MeOH和硫酸锌预处理以沉淀蛋白质，然后直接注入HPLC系统。用Puresil C18色谱柱（粒径5 / im）进行色谱分离，流动相由0.1m乙酸铵缓冲液（pH 5.5）和乙腈（70/30，v / v）组成，其中含20d -溴化戊铵。用分光荧光计（激发波长380nm，发射波长540nm）监测柱流出物。该方法对SN-38内酯（5-2500 ng / ml）和羧酸盐（5-1000 ng / ml）有效。

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