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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Axodendritic fibres of mouse cerebellar granule neurons exhibit a diversity of functional P2X receptors.
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Axodendritic fibres of mouse cerebellar granule neurons exhibit a diversity of functional P2X receptors.

机译：小鼠小脑颗粒神经元的轴突纤维表现出多种功能性P2X受体。

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摘要

Distribution and functional expression of P2X receptors were analyzed in mouse cerebellum axodendritic fibres, using different experimental approaches such as RT-PCR, western blot, immunochemistry, microfluorimetric experiments and exocytotic studies. RT-PCR and western blot demonstrated the presence of P2X1-4,7 subunits in both whole cerebellum and mouse cerebellar granule cultured neurons. Immunochemistry analysis of tissular and cellular location of P2X1-4,7 receptors confirmed their presence and unequal distribution between somas and axodendritic prolongations. Microfluorimetric experiments using a variety of modulators of the P2X subunits revealed the presence of different functional P2X receptors in the axodendritic fibres. The use of the synthetic agonist alpha,beta-meATP and the antagonist Ip(5)I revealed the activation of functional P2X1 and P2X3 receptors. Responses mediated by P2X1 subunits were also confirmed by using ZnSO(4). Activation of functional P2X4 receptors is observed when stimulated in the presence of ivermectin. Exocytotic studies confirmed the role of most P2X subunits in the activation of neurotransmitter release in axodendritic fibres from mouse cerebellar granule neurons.
机译：使用不同的实验方法，例如RT-PCR，蛋白质印迹，免疫化学，微荧光实验和胞吐研究，分析了小鼠小脑轴突纤维中P2X受体的分布和功能表达。 RT-PCR和蛋白质印迹证明了在整个小脑和小鼠小脑颗粒培养的神经元中都存在P2X1-4,7亚基。对P2X1-4,7受体的组织和细胞位置进行的免疫化学分析证实了它们的存在以及它们在躯体和轴突延伸之间的分布不均。使用多种P2X亚基调节剂的微荧光实验显示，在腋突纤维中存在不同功能的P2X受体。合成激动剂α，β-meATP和拮抗剂Ip（5）I的使用揭示了功能性P2X1和P2X3受体的激活。通过使用ZnSO（4）也证实了P2X1亚基介导的反应。在伊维菌素存在下刺激时，观察到功能性P2X4受体的激活。细胞外研究证实了大多数P2X亚基在激活小鼠小脑颗粒神经元轴突纤维中神经递质释放中的作用。

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