• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Characterization of two novel tritiated radioligands for labelling Neuropeptide FF (NPFF(1) and NPFF(2)) receptors.
【24h】

Characterization of two novel tritiated radioligands for labelling Neuropeptide FF (NPFF(1) and NPFF(2)) receptors.

机译：用于标记神经肽FF（NPFF（1）和NPFF（2））受体的两个新型tri化放射性配体的表征。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The binding characteristics of [(3)H]-NPVF and [(3)H]-EYF, the two first tritiated probes for the respective labelling of NPFF(1) and NPFF(2) receptors, are presented. In membranes from CHO cells transfected with the human NPFF(1) receptor, [(3)H]-NPVF labelled one class of binding sites with a high affinity (Bmax=4pmol/mg protein, Kd=2.65nM). In membranes from CHO cells transfected with the human NPFF(2) receptor, [(3)H]-EYF labelled one class of binding sites with a high affinity (Bmax=16pmol/mg protein, Kd=0.54nM). Both radioligands exhibited time-dependent binding, low (10-20%) non-specific binding and poor cross-reactivity towards the related receptor subtype. The potency of different NPFF ligands to displace [(3)H]-NPVF and [(3)H]-EYF binding profiles was in good agreement with the profile previously measured by using (125)I-probes (NPFF(1) receptor: NPVF> or =1DMe=SPA-NPFF>NPFF=SQA-NPFF=QFW-NPSF>NPSF>RF9; NPFF(2) receptor: SPA-NPFFSQA-NPFF=QFW-NPSF=1DMe=NPFFNPSF=NPVF>RF9). Therefore, [(3)H]-NPVF and [(3)H]-EYF are new valuable tools for performing binding on NPFF receptors.
机译：介绍了[（3）H] -NPVF和[（3）H] -EYF的结合特性，这两个是分别标记NPFF（1）和NPFF（2）受体的第一个tri化探针。在用人NPFF（1）受体转染的CHO细胞膜中，[（3）H] -NPVF标记了一类具有高亲和力的结合位点（Bmax = 4pmol / mg蛋白，Kd = 2.65nM）。在用人NPFF（2）受体转染的CHO细胞膜中，[（3）H] -EYF以高亲和力标记了一类结合位点（Bmax = 16pmol / mg蛋白，Kd = 0.54nM）。两种放射性配体均表现出时间依赖性结合，低（10-20％）非特异性结合以及对相关受体亚型的交叉反应性差。不同NPFF配体取代[（3）H] -NPVF和[（3）H] -EYF结合谱的能力与先前使用（125）I-probes（NPFF（1）receptor ：NPVF>或= 1DMe = SPA-NPFF> NPFF = SQA-NPFF = QFW-NPSF> NPSF> RF9; NPFF（2）受体：SPA-NPFF SQA-NPFF = QFW-NPSF = 1DMe = NPFF NPSF = NPVF> RF9）。因此，[（3）H] -NPVF和[（3）H] -EYF是在NPFF受体上进行结合的新的有价值的工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号