...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Inhibition of Rho kinase by hydroxyfasudil attenuates brain edema after subarachnoid hemorrhage in rats
【24h】

Inhibition of Rho kinase by hydroxyfasudil attenuates brain edema after subarachnoid hemorrhage in rats

机译：羟基法舒地尔对Rho激酶的抑制作用可减轻大鼠蛛网膜下腔出血后脑水肿

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The blood-brain barrier (BBB) disruption and brain edema are important pathophysiologies of early brain injury after subarachnoid hemorrhage (SAH). This study is to evaluate whether Rho kinase (Rock) enhances BBB permeability via disruption of tight junction proteins during early brain injury. Adult male rats were assigned to five groups; Sham-operated, SAH treated with saline, a Rock inhibitor hydroxyfasudil (HF) (10 mg/kg) treatment at 0.5 h after SAH, HF treatment at 0.5 and 6 h (10 mg/kg, each) after SAH, and another Rock inhibitor Y27632 (10 mg/kg) treatment at 0.5 h after SAH. The perforation model of SAH was performed and neurological score and brain water content were evaluated 24 and 72 h after surgery. Evans blue extravasation, Rock activity assay, and western blotting analyses were evaluated 24 h after surgery. Treatment of HF significantly improved neurological scores 24 h after SAH. Single treatment with HF and Y27632, and two treatments with HF reduced brain water content in the ipsilateral hemisphere. HF reduced Evans blue extravasation in the ipsilateral hemisphere after SAH. Rock activity increased 24 h after SAH, and HF reversed the activity. SAH significantly decreased the levels of tight junction proteins, occludin and zonula occludens-1 (ZO-1), and HF preserved the levels of occluding and ZO-1 in ipsilateral hemisphere. In conclusion, HF attenuated BBB permeability after SAH, possibly by protection of tight junction proteins.
机译：血脑屏障（BBB）的破坏和脑水肿是蛛网膜下腔出血（SAH）后早期脑损伤的重要病理生理。这项研究旨在评估Rho激酶（Rock）在早期脑损伤期间是否通过破坏紧密连接蛋白来增强BBB通透性。成年雄性大鼠分为五组。假手术，用盐水进行SAH处理，在SAH后0.5 h进行Rock抑制剂羟基法舒地尔（HF）（10 mg / kg）处理，在SAH之后0.5和6 h进行HF治疗（每次10 mg / kg）和另一块Rock SAH后0.5小时治疗Y27632（10 mg / kg）抑制剂。进行SAH穿孔模型，并在术后24和72 h评估神经学评分和脑含水量。术后24小时评估伊文思蓝外渗，岩石活性测定和蛋白质印迹分析。 HF的治疗可显着改善SAH后24小时的神经学评分。 HF和Y27632的单次治疗，以及HF的两次治疗可降低同侧半球的脑含水量。 HF减少了SAH后同侧半球的Evans蓝色外渗。 SAH后24小时，岩石活动增加，而HF使活动反向。 SAH显着降低了紧密连接蛋白，occludin和zonula occludens-1（ZO-1）的水平，并且HF保留了同侧半球的occlusion和ZO-1的水平。总之，HF可能通过保护紧密连接蛋白来减弱SAH后的BBB通透性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号