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首页> 外文期刊>Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry >Fast axonal transport of the vesicular acetylcholine transporter (VAChT) in cholinergic neurons in the rat sciatic nerve.
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Fast axonal transport of the vesicular acetylcholine transporter (VAChT) in cholinergic neurons in the rat sciatic nerve.

机译：大鼠坐骨神经胆碱能神经元中水泡乙酰胆碱转运蛋白（VAChT）的轴突快速转运。

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摘要

The sciatic nerve, as a part of the peripheral nervous system (PNS), has been used to study axonal transport for decades. It contains motor, sensory as well as autonomic axons. The present study has concentrated on the axonal transport of the synaptic vesicle acetylcholine transporter (VAChT), using the "stop-flowerve crush" method. After blocking fast axonal transport by means of a crush, distinct accumulations of various synaptic vesicle proteins, including VAChT, and peptides developed during the first hour after crush-operation and marked increases were observed up to 8 h post-operative. Semiquantitative analysis, using cytofluorimetric scanning (CFS) of immuno-incubated sections, revealed a rapid rate of accumulation proximal to the crush, and that the ratio between distal accumulations (organelles in retrograde transport) and proximal accumulations (organelles in anterograde transport) was about 40%. Most synaptic vesicle proteins were colocalized in the axons proximal to the crush. VAChT-immunoreactive axons were also immunoreactive for choline acetyltransferase (ChAT). Autonomic axons with VAChT also contained VIP-LI. The results demonstrate (1) that VAChT, as well as other synaptic vesicle proteins, is transported with fast axonal transport in motor axons as well as in autonomic post-ganglionic neurons in this nerve, (2) VAChT colocalized in motor axons with SV2 as well as with synaptophysin, indicating storage in the same axonal particle, (3) in the autonomic postganglionic sympathetic cholinergic fibres, VAChT colocalized with VIP, but VIP-LI was present in rather large granular structures while VAChT-LI was present mostly as small granular elements, (4) in motor as well as in autonomic axons ChAT-LI was present in VAChT-positive axons, and (5) the ratio of recycling (retrogradely accumulated) VAChT-IR was about 40%, in contrast to the recycling fraction of synaptophysin that was about 70%.
机译：坐骨神经作为周围神经系统（PNS）的一部分，几十年来一直被用于研究轴突运输。它包含运动，感觉以及自主神经轴突。本研究集中在突触囊泡乙酰胆碱转运蛋白（VAChT）的轴突运输，使用“停止流/神经挤压”方法。在通过压迫阻断快速轴突运输后，各种突触小泡蛋白（包括VAChT）和压迫手术后第一小时内形成的肽明显积累，并在术后8小时内观察到明显增加。使用免疫孵育切片的细胞荧光扫描（CFS）进行的半定量分析显示，挤压物附近的堆积速度很快，远端堆积（逆行运输中的细胞器）与近端堆积（顺行运输中的细胞器）之间的比率约为40％。大多数突触小泡蛋白共定位在挤压的近端轴突中。 VAChT免疫反应性轴突对胆碱乙酰基转移酶（ChAT）也具有免疫反应性。带有VAChT的自主神经轴突也包含VIP-LI。结果表明（1）VAChT和其他突触小泡蛋白在运动轴突以及该神经的自主神经节后神经元中以快速轴突运输方式运输，（2）VAChT与SV2作为共定位在运动轴突中以及与突触素一起使用，表明储存在相同的轴突颗粒中，（3）在自主神经节后交感性胆碱能纤维中，VAChT与VIP共定位，但VIP-LI以相当大的颗粒状结构存在，而VAChT-LI则以小颗粒状存在元素，（4）在马达及自主神经轴突中，ChAT-LI存在于VAChT阳性轴突中，（5）VAChT-IR的回收率（逆行累积）约为40％，与回收率相比突触素的比例约为70％。

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